Examination of hearing impairment and vestibular dysfunction associated with streptozotocin

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K18309
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56050:Otorhinolaryngology-related
• Research Institution: Nagoya University
• Principal Investigator: Sugimoto Satofumi 名古屋大学, 医学部附属病院, 助教 (30759668)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: Streptozotocin / 聴力 / 前庭機能 / ABR / DPOAE / 糖尿病 / マウス

Outline of Final Research Achievements

We used C57BL6 / J mice. We had performed auditory brainstem response (ABR), distortion component otoacoustic emission (DPOAE) and balance test 4 weeks after intratympanic injection of streptozotocin (STZ), gentamicin (GM) and saline. Compared to the saline group, ABR results were significantly deteriorated at frequencies of 4 kHz to 32 kHz in the STZ and GM groups. DPOAE amplitudes were also significantly deteriorated at frequencies of 5.6 kHz to 22 kHz in the STZ group and 5.6 kHz to 16 kHz in the GM group. Balance disorder was observed only in the GM group.

Free Research Field

耳鼻咽喉科学

Academic Significance and Societal Importance of the Research Achievements

STZ鼓室内投与により聴力障害が誘発されることは報告例がなく、本研究により初めて基礎データが得られた。聴力障害所見の中でも、主に蝸牛の外有毛細胞活性を反映するDPOAEの悪化を認めるため、STZ鼓室内投与により蝸牛障害が生じることが示唆された。また、STZ鼓室内投与により前庭機能障害は誘発されないことも示唆された。今後は、例数を増やすと共に、他の系統のマウスでの測定も実施して、慎重に検証を重ねる予定である。