2023 Fiscal Year Final Research Report
Damage and Repair of Barrier Function in Cultured Vocal Fold Mucosa
| Project/Area Number |
20K18311
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 56050:Otorhinolaryngology-related
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | 声帯 / バリア機能 / 酸傷害 |
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| Outline of Final Research Achievements |
We mimicked acid-induced epithelial barrier dysfunction in vocal folds in an in vitro environment to observe damage patterns and the recovery process. Cultured vocal fold mucosal epithelial cells were exposed to an acidic medium for a set period, and transepithelial electrical resistance (TEER) was measured to confirm a transient decrease and subsequent recovery in resistance. Adding pepsin, an enzyme present in the stomach, to the acidic medium further decreased resistance. Scanning electron microscopy revealed that the reduction in microvilli on the cell surface was similar between acid injury alone and acid injury with pepsin. The results indicate that epithelial barrier dysfunction varies by the type of injury.
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| Free Research Field |
耳鼻咽喉科
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| Academic Significance and Societal Importance of the Research Achievements |
粘膜上皮は深層組織を外部刺激から守る物理的障壁（上皮バリア）としての機能を持った重要な構造であり、声帯粘膜においては乾燥、タバコ、胃酸、手術、音声酷使などがバリア機能の傷害因子として指摘されている一方で機能の修復について着目した報告は未だ存在しない。様々な要素が複合して粘膜のバリア機能は構築されているが、本研究では逆流性食道炎による胃酸暴露環境にともなう傷害を培養環境で再現した。本研究の結果、傷害の種類により機能不全に陥る部位に違いが有ることがわかった。バリア機能の充分な評価には様々な傷害手技と多面的な評価手法が必要であることが重要であることが示唆された。
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