Elucidation of the regulatory mechanism of RP by regulating PAPR1 activity

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K18384
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56060:Ophthalmology-related
• Research Institution: Yamaguchi University
• Principal Investigator: Ashimori Atsushige 山口大学, 大学院医学系研究科, 助教 (60870459)
• Project Period (FY): 2020-04-01 – 2022-03-31
• Keywords: Aging / PARP1 / NAD+

Outline of Final Research Achievements

The purpose of this study was to elucidate the regulatory mechanisms of retinitis pigmentosa (RP) by regulating PARP1 activity.
This study identified PARP1 target factors by LC-MS/MS that may be involved in RP, and these need to be validated in more detail. We also found that PARP1 activation is differentially regulated by PARP1 activators or inhibitors within eye-derived cells, which may help elucidate the regulatory mechanism of PARP1 in RP progression.

Free Research Field

眼科学関連

Academic Significance and Societal Importance of the Research Achievements

RPの原因遺伝子及び機序、またはRP進行を遅らせる治療薬の研究などは広く行われてきた一方で、RPとPARP1活性制御を結びつける本研究は新規性が高い。PAR化タンパク質の同定では、RP制御に関与する直接的な因子を発見するには至らなかったものの、近年報告されている手法を用いることにより、ブレイクスルーとなる潜在性がある。また、本研究で予想外にえられたPARP1の活性制御機構は、RP制御の枠を超えPARP1の新たな制御機構および分子機序を明らかにできる可能性がある。