Development of a novel treatment for lower limb ischaemia via promotion of fat stem cell regenerative capacity by fucoidan.

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K18412
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56070:Plastic and reconstructive surgery-related
• Research Institution: Tottori University
• Principal Investigator: MORITA Maki 鳥取大学, 医学部附属病院, 医員 (60838407)
• Project Period (FY): 2020-04-01 – 2022-03-31
• Keywords: 血流不全 / フコイダン / 脂肪組織由来幹細胞 / モズク / アカモク

Outline of Final Research Achievements

The angiogenic effects of mozuku HMWF were compared with those of red mullet HMWF to determine the effects of pre- and post-ischemic treatment with mozuku HMWF or red mullet HMWF on blood flow in the ischaemic limb. The LDBF of the control group, the mozuku HMWF-treated group before and after ischaemia, and the akamoku HMWF-treated group before and after ischaemia were analysed. The LDBF of the mozuku group was higher than that of the control and akamoku groups, and similar results were obtained when comparing the effect of pre- and post-iscukaemic administration of mozuku HMWF and akamoku HMWF on capillary density in the ischaemic limb by immunostaining muscle capillaries of the ischaemic limb on day 28 with anti-CD31 antibody. Comparison of the pre- and post-iscovery group with the post-iscovery only group showed a clear recovery of blood flow in the pre- and post-iscovery group.

Free Research Field

形成外科

Academic Significance and Societal Importance of the Research Achievements

加齢をはじめ，喫煙や糖尿病など生活習慣病をリスクファクターとするPADの治療法としては主に抗血小板薬などを用いた薬物療法，血管内治療，バイパス術などの手術療法が選択されているが、いずれも高侵襲であり治療が奏功しないケースも数多く存在する。元々効果が知られている再生医療の一つである脂肪組織由来幹細胞の使用や健康食品として知られるフコイダンを摂取することによって血流改善が得られることが証明できたことは、より低侵襲で血流不全の諸症状に対し対処できる方法が存在することが明らかとなったという意味で、非常に意義深い研究であったと考える。