Development of new therapies for cutaneous radiation injury using in vivo lineage tracing of adipose-derived stem cells

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K18441
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56070:Plastic and reconstructive surgery-related
• Research Institution: Jichi Medical University
• Principal Investigator: Morishita Yuya 自治医科大学, 医学部, 臨床助教 (60860872)
• Project Period (FY): 2020-04-01 – 2022-03-31
• Keywords: 放射線潰瘍

Outline of Final Research Achievements

By mating Prx1Cre mice with Ai14 mice and Col1 / EGFP mice, genetically modified mice (PAG mice) in which cells derived from Prx1-expressing cells permanently express tdTomato and type I collagen-expressing cells express EGFP. Was produced. Fat-derived stem cells (tdT-ADSCs) were extracted and cultured from the inguinal fat of PAG mice, and injected and transplanted into the skin radiation-damaged area created on the back of Wild mice. When the tissue after transplantation was observed by fluorescent immunostaining, tdTomato-positive GFP-positive cells were observed under the skin, indicating that the transplanted tdTomato-positive ADSCs contributed to collagen production in the process of repairing radiation injury.

Free Research Field

形成外科学

Academic Significance and Societal Importance of the Research Achievements

本研究の目的は、脂肪由来幹細胞を用いた皮膚放射線障害に対する再生治療を開発することであった。マウスの疾患モデルに対して移植した脂肪由来幹細胞の生体内における局在・形質変化を系譜追跡という手法を用いて追跡することにより、従来の手法では不可能であった脂肪由来幹細胞の生体内における挙動の一端が解明され、臨床応用の足がかりが出来た。