Role of operon containing genes related to type IX secretion system of periodontal pathogens and involvement in pathogenicity

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K18475
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57020:Oral pathobiological science-related
• Research Institution: Okayama University
• Principal Investigator: ONO Shintaro 岡山大学, 歯学部, 客員研究員 (80866517)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: Porphyromonas gingivalis / IX型分泌装置 / ジンジパイン

Outline of Final Research Achievements

Porphyromonas gingivalis is involved in the onset and progression of periodontal disease by secreting the protease gingipain through the type IX secretion system. PGN_0296 operon is involved in the type IX secretion system but its constituent gene PGN_0296 did not have a direct effect on the type IX secretion system. PGN_0298 was speculated an essential gene in P. gingivalis. It was suggested that the PGN_0301 gene product is an outer membrane protein with chaperone activity that modifies outer membrane proteins including gingipains.

Free Research Field

口腔微生物学

Academic Significance and Societal Importance of the Research Achievements

Porphyromonas. gingivalis は歯周組織に定着，増殖しIX型分泌装置を通してプロテアーゼであるジンジパインを分泌することで，歯周病の病態に大きく影響を与える。さらに歯周病の進行は糖尿病や循環器疾患など全身性疾患にも関わっている。PGN_0296オペロンはIX型分泌装置に関わっており，その構成遺伝子であるPGN_0298は必須遺伝子の可能性があり，PGN_0301はジンジパインを含む外膜蛋白質を修飾するシャペロニン活性を有する外膜タンパク質であることが示唆され，ジンジパイン分泌の機構がまた解析されたことで，歯周病予防・治療への一助となったと思われる。