2021 Fiscal Year Final Research Report
Are membrane vesicles of periodontopathogenic bacteria a pathogenic factor in Alzheimer's disease?
| Project/Area Number |
20K18492
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 57020:Oral pathobiological science-related
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| Research Institution | Niigata University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2022-03-31
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| Keywords | メンブレンベシクル / 歯周病原細菌 / Porphyromonas gingivalis / アルツハイマー病 / グリシン |
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| Outline of Final Research Achievements |
To investigate the possible role of membrane vesicles of the periodontopathogenic bacterium Porphyromonas gingivalis in the pathogenesis of Alzheimer's disease, P. gingivalis vesicles were prepared and used in an in vitro study using a blood-brain barrier model. In order to efficiently conduct the study, we investigated the conditions under which the production of bacterial vesicles increases. The vesicle yield was significantly increased when glycine was added during culture using Escherichia coli as a model. Compared to non-induced vesicles, endotoxin activity was decreased, but the same immunoinductive activity was maintained. Glycine induction was also effective in Acinetobacter baumannii.
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| Free Research Field |
細菌学
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| Academic Significance and Societal Importance of the Research Achievements |
アルツハイマー病は発症の原因が特定されておらず，認知症を根治できる薬物療法も存在しない．したがって治療が困難であるため，アルツハイマー病の予防や進行を遅らせることは極めて重要である．したがって，Porphyromonas gingivalisとアルツハイマー病との関連性を明らかにできれば，アルツハイマー病の発症メカニズムの理解や予防に貢献できる．また，グリシンによる細菌のメンブレンベシクル産生誘導法は，感染症をはじめとしてメンブレンベシクルが関連する様々な研究の進展に役立てられ，アジュバントやワクチン開発等の幅広い分野への応用展開も期待することができる．
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