2022 Fiscal Year Final Research Report
identification of M2 macrophage-secreted factors involved in oral cancer relapse
Project/Area Number |
20K18733
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 57060:Surgical dentistry-related
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Research Institution | Yokohama City University |
Principal Investigator |
OKUBO Makiko 横浜市立大学, 医学研究科, 客員研究員 (10780611)
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Keywords | 口腔癌 |
Outline of Final Research Achievements |
Regulation of relapse following radiotherapy is important for the improvement of prognosis in oral cancer treatment. We previously found that recruitment of M2 type macrophage contributed to relapse of tumor after radiation. Although M2 macrophage is thought to be pro-tumorigenic, the role and molecular mechanism of M2 macrophage in tumor microenvironment in recurrence is not known yet. Therefore, the subject of this study was to clarify the role of M2 macrophage in relapse of oral cancer using organoid culture, in which the tumormicroenvironment are closely mimic in vitro. We cocultured several different types of cells including cancer, endothelial, stroma, and monocytes for establishment of oral cancer organoid. We found that the resistance against chemo drugs was seen in 3D-organoid compared to either oral cancer cells alone or 2D-cocultured cells. The conditioned media from M2 macrophage induced angiogenesis in vitro assay.
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Free Research Field |
口腔癌研究
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Academic Significance and Societal Importance of the Research Achievements |
まだ最終的な成果は得られていないが、本研究成果から口腔がん治療における最も重要な問題の一つである治療後に生じる再発におけるM2マクロファージの役割の一端が明らかとなった。今後これに対する治療標的や診断方法が確立されることにより新たな治療法の開発や診断ツールの開発への貢献が期待され、学術的意義のみならず、社会的な意義においても重要となりうる。
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