Analysis of the swallowing activity in the central nervous system by using newly developed medullary slice preparation

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K18746
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57060:Surgical dentistry-related
• Research Institution: Osaka University
• Principal Investigator: Kondo Takahide 大阪大学, 大学院歯学研究科, 招へい教員 (90870444)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 嚥下活動 / セントラルパターンジェネレーター / 呼吸活動 / 電気生理学

Outline of Final Research Achievements

We reported the use of a transverse brainstem slice(800μm) to study the activity of the central pattern generator(CPG) responsible for the swallowing. Using electrophysiological recording from the XII nerve, we demonstrated that certain bursts evoked by the electrical stimuli applied to the X or application of the bicuculline represent the discharges of this pattern generator. In addition, we showed the effects of NMDA and non-NMDA receptor antagonists in the swallowing-like activity.

Free Research Field

電気生理学

Academic Significance and Societal Importance of the Research Achievements

嚥下活動を発現しうる延髄スライス標本の作成に成功した。この標本の作成に成功したのは本研究が世界で初であり、今後この実験方法を用いたさまざまな中枢神経系のネットワーク解析が進むことが期待できる。