Investigation of the development of pain during orthodontic tooth movement with adenosine triphosphate

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K18789
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57070:Developmental dentistry-related
• Research Institution: Kyushu Dental College
• Principal Investigator: Mizuhara Masahiro 九州歯科大学, 歯学部, 助教 (60845402)
• Project Period (FY): 2020-04-01 – 2022-03-31
• Keywords: 歯科矯正学 / アデノシン三リン酸 / 小胞型ヌクレオチドトランスポーター / クロドロン酸 / ビスホスホネート / 疼痛

Outline of Final Research Achievements

Orthodontic patients experience pain and discomfort to varying degrees during the course of treatment. Therefore, pain control is imperative in orthodontic treatment. In the present study, we examined whether the vesicular nucleotide transporter (VNUT), which is a vesicle transporter of adenosine triphosphate (ATP), in periodontal ligament cells participates in tooth movement-induced nociception.
A fixed nickel-titanium alloy closed-coil spring appliance was constructed to induce mesial movement of the right maxillary first molar in rats. face-grooming behaviors (indicators of nociception) were significantly increased after experimental tooth movement. Increased face-grooming behaviors were suppressed by systemic administration of the inhibitor. However, Changes in expression of VNUT gene was not detected.

Free Research Field

歯科矯正学

Academic Significance and Societal Importance of the Research Achievements

本研究の結果から、矯正学的歯の移動における疼痛発症にATPの小胞輸送体であるVNUTが重要な役割を果たしていることが示された。歯根膜組織におけるVNUTの役割についての詳細な解明は出来なかったものの、広く存在していることから、様々な情報伝達に関与している可能性がある。疼痛発症だけでなく、骨リモデリングの細胞間情報伝達や機序の解明への今後の研究の一助になると思われる。