2023 Fiscal Year Final Research Report
Analysis of new function of nucleic acid in adipose tissue inflammation
| Project/Area Number |
20K19695
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 59040:Nutrition science and health science-related
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| Research Institution | Konan Women's University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | 慢性炎症 / 肥満 / 脂肪組織 / 自己遊離核酸 / インスリン抵抗性 / DNA |
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| Outline of Final Research Achievements |
Chronic inflammation centered macrophages in adipose tissue participates the development of insulin resistance. It is suggested relationship between insulin resistance and cell free DNA, which is one of the molecules released from damaged cells triggered by stress such as over nutrition. This study aimed to explore the relationship between insulin resistance and the mechanism of recognizing and breaking down DNA.
Measuring activities of DNase Ⅱ in visceral adipose tissue in obese mice revealed that increased DNase Ⅱ activity in obese adipose tissue. The results of in vitro experiments revealed that TLR agonists or cGAMP affected mRNA expression of Trex 1 and molecules associated STING.
These results suggested that the mechanism of recognizing and breaking down DNA may involved in the mechanism of the development of insulin resistance and chronic inflammation thorough macrophages in visceral adipose tissue.
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| Free Research Field |
代謝栄養学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により、脂肪組織のインスリン抵抗性とマクロファージを介した炎症性質の発現に複数の核酸認識・分解機構が関与する可能性が示唆された。今後、脂肪組織の慢性炎症進展とDNA認識制御および分解能の関連性を、シグナル伝達機構を含め詳細に明らかにすることで、全身の代謝状態が問題となる生活習慣病に対する新たな予防・治療方法の開発への応用が期待される。cfDNAが関わる病態には、血管の慢性炎症である動脈硬化症にも関与することが示唆されており、全身の代謝調節に関わる核酸の栄養生理学的意義を明らかにすることで、生活習慣病に対する新たな予防・治療方法の開発への応用が期待される
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