2022 Fiscal Year Final Research Report
Function-based environmental microbial analysis to selectively predict microbial dynamics
Project/Area Number |
20K19964
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 63010:Environmental dynamic analysis-related
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Research Institution | National Institute of Advanced Industrial Science and Technology |
Principal Investigator |
Aoyagi Tomo 国立研究開発法人産業技術総合研究所, エネルギー・環境領域, 主任研究員 (10812761)
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Keywords | 廃水処理 / 汚泥微生物群集 / 嫌気消化 / 酢酸酸化 / 有機酸 / 共生細菌 / メタン生成菌 / 高感度SIP |
Outline of Final Research Achievements |
Anaerobic digester was fed with synthetic wastewater and operated for 467 days. The operating conditions were temporarily changed to observe the environmental response of the microorganisms. 13C-acetate or 13C-propionate was added to the sludge, and high-sensitivity rRNA-SIP (Stable Isotope Probing) were applied. As a result, novel acetate- and propionate-oxidizing bacteria and methanogens were identified. Dynamics data of the microorganisms identified by the high-sensitivity rRNA-SIP were extracted throughout the reactor operation period. The results showed that methanogens decreased in abundance during events when volatile fatty acids (VFAs) accumulated. Most of the acetate-oxidizing bacteria decreased in abundance at this time, while some propionate-oxidizing bacteria decreased in abundance while others did not.These results suggest that each VFAs-oxidizing bacterium has a different degree of association with methanogens.
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Free Research Field |
環境微生物
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Academic Significance and Societal Importance of the Research Achievements |
人工環境における微生物の仲介するプロセスとして最大の発展を遂げている汚泥を用いた廃水処理は、世界中で利用されている。しかし、汚泥を構成する微生物は、その実体を把握されないままで、現場の経験や勘に頼ったプロセスの運用がなされてきた。本研究では、創エネ型の廃水処理である「嫌気性廃水処理」をラボスケールで長期間実施し、安定処理と不安定化の際の各種データを蓄積した。その結果、プロセスの安定・高効率処理に重要な未知微生物群の同定とそれらの生育特性を見出した。本研究成果は廃水処理の高精度な性能予測および汚泥微生物の人為制御に向けた重要な知見となる。
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