2021 Fiscal Year Final Research Report
Simple and Rapid metabolite analysis of crops by new mass spectrometry PESI/MS/MS
| Project/Area Number |
20K20372
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| Project/Area Number (Other) |
18H05361 (2018-2019)
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| Research Category |
Grant-in-Aid for Challenging Research (Pioneering)
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| Allocation Type | Multi-year Fund (2020)
Single-year Grants (2018-2019) |
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| Review Section |
Medium-sized Section 39:Agricultural and environmental biology and related fields
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
財津 桂 名古屋大学, 医学系研究科, 准教授 (30700546)
林 由美 名古屋大学, 医学系研究科(保健), 講師 (30632707)
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| Project Period (FY) |
2018-06-29 – 2022-03-31
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| Keywords | メタボローム解析 / 農作物 / 植物 / PESI/MS/MS / 探針エレクトロスプレー/タンデム質量分析 / 非破壊・リアルタイム分析 / アントシアニン |
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| Outline of Final Research Achievements |
In this study, we showed that the new mass spectrometry PESI/MS/MS (Probe Electrospray Ionization-Tandem Mass Spectrometry) is a rapid, laborless and useful technology for the metabolite analysis of plants. By PESI/MS/MS, metabolites can be analyzed in seconds by simply sticking a needle into the sample, and local metabolite analysis and imaging analysis are also available. In addition, we developed the method to analyze 130 metabolites in 5 minutes and the platform to process the data automatically within 1 minute.
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| Free Research Field |
園芸生理・生化学
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| Academic Significance and Societal Importance of the Research Achievements |
人類は植物の代謝物を食料や栄養源として，また医薬品や工業原料，エネルギー原料として活用してきた．近年では，植物の代謝物を質量分析装置で分析することが多いが，その分析には多くの手間と時間を必要とする．本研究では，微細な針を試料に刺すだけで，秒オーダーで代謝物分析できる，新しい質量分析技術PESI/MS/MS（Probe Electrospray Ionization-Tandem Mass Spectrometry）が，植物の代謝物解析に有効であることを世界で初めて明らかにした．
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