Development of strigolactone analogues capable of modifying the composition of the arbuscular mycorrhizal fungal community

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K20452
• Project/Area Number (Other): 19H05537 (2019)
• Research Category: Grant-in-Aid for Challenging Research (Pioneering)
• Allocation Type: Multi-year Fund (2020); Single-year Grants (2019)
• Review Section: Medium-sized Section 38:Agricultural chemistry and related fields
• Research Institution: Osaka Metropolitan University (2022); Osaka Prefecture University (2019-2021)
• Principal Investigator: Akiyama Kohki 大阪公立大学, 大学院農学研究科, 教授 (20285307)
• Project Period (FY): 2019-06-28 – 2023-03-31
• Keywords: アーバスキュラー菌根菌 / ストリゴラクトン / 菌根菌叢

Outline of Final Research Achievements

The aim of this study was to develop a technique for modifying the composition of arbuscular mycorrhizal fungal communities using synthetic analogues of non-canonical strigolactone (SL). Various analogues of methyl carlactonoate (MeCLA) with a para-substituted benzene in the A-ring were newly synthesized. It was found that the hyphal branching activity in germinating spores of the AM fungus G. margarita could be regulated by varying the carbon chain length of the alkyl substituent. In a fungal composition assay using a synthetic community involving three AM fungi, Rhizophagus irregularis, R. clarus, and G. margarita, several analogues showed a modifying effect on the fungal community, significantly reducing R. irregularis and increasing R. clarus compared to the control.

Free Research Field

天然物化学・生物有機化学

Academic Significance and Societal Importance of the Research Achievements

フィールド条件では植物種によって共生している菌根菌叢におけるAM菌の菌種組成が異なるが、その機構についてはほとんど分かっていない。また、共生効果の高い有用なAM菌を外来的に導入する場合に、土着菌による先住者効果が障害となっている。本研究で開発した非典型的SLアナログは、3種のAM菌からなる合成コミュニティーにおいて、同属異種の2つのAM菌の菌叢組成比を逆転させることができた。本アナログは、菌根菌叢形成の分子機構の解明に利用できるだけでなく、菌叢改変剤としての応用展開が期待できる。