2023 Fiscal Year Final Research Report
Analysis on epidermal differentiation by amniotic fluid component
| Project/Area Number |
20K20568
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| Research Category |
Grant-in-Aid for Challenging Research (Pioneering)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 38:Agricultural chemistry and related fields
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
村井 篤嗣 名古屋大学, 生命農学研究科, 教授 (10313975)
大蔵 聡 名古屋大学, 生命農学研究科, 教授 (20263163)
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| Project Period (FY) |
2020-07-30 – 2024-03-31
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| Keywords | 表皮 / 立体培養 / ケラチノサイト / 低酸素誘導因子 / 羊水 / トランスグルタミナーゼ |
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| Outline of Final Research Achievements |
Epidermal structure can be reproduced by three-dimensional cultured system. In this case, air-exposure treatment is inevitable for keratinocyte differentiation. However, the mechanism by which air/liquid culture system enables the stimulation. We investigated gene and protein expression patterns between the air-exposure and the submerged cultures, by biochemical and molecular biological analyses. Then we clarified the HIF (hypoxia-inducible factor) is responsible for differentiation. We revealed its higher transcriptional activity hampers differentiation although the higher levels of protein expression is observed in the case of air-exposure culture.
In parallel, we assumed the keratinocyte differentiation factor exits in the amniotic fluid because embryo skin formation was achieved without air-exposure. By purification from goat, mice, and chicken egg amniotic fluid, we identified proteins that enable differentiation. Biochemical analyses of these proteins are in progress.
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| Free Research Field |
応用分子生命科学
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| Academic Significance and Societal Importance of the Research Achievements |
表皮立体培養系は、皮膚表皮構造を模倣できるため、再生医療や創薬、化粧品研究などでも用いられている。しかしこのシステムの空気暴露を必須とする理由はまだ不明である。今回その機構について多くの知見を得た結果、従来の液性因子に加えて低酸素応答機構の関与を示した。この点を考慮して空気暴露が無い状態での再生系を確立し、様々な成長因子の添加や表皮に本来存在する細胞の共存培養の可能性を示した。羊水中から多数の表皮分化促進能を持つタンパク質画分を精製し、候補分子を同定した。本研究の成果は、表皮形成の応用生物化学的な解析による基本的知見の蓄積に加え、産業応用や畜産学研究にも資するものである。
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