2021 Fiscal Year Research-status Report
A Novel Mechanism of Genetic Mutation Independent Metal Tolerance in a Multi-Hierarchical Terrestrial Organism Population Using High Precision XAFS
| Project/Area Number |
20K20633
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| Research Institution | Hokkaido University |
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Principal Investigator |
中山 翔太 北海道大学, 獣医学研究院, 准教授 (90647629)
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| Co-Investigator(Kenkyū-buntansha) |
藤森 崇 龍谷大学, 先端理工学部, 准教授 (20583248)
中田 北斗 北海道大学, 獣医学研究院, 博士研究員 (60815273) [Withdrawn]
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| Project Period (FY) |
2020-07-30 – 2023-03-31
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| Keywords | 金属耐性機構 / 動物種差 / XAFS解析 / 野生動物 / 毒性発現 / カブウェ鉱床 |
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| Outline of Annual Research Achievements |
野生ラットの臓器および内容物の鉛化学形態分布について、Pb L3端 XANESスペクトルによる解析を進めた。高濃度の鉛で汚染されたラットであることに加えて、凍結乾燥による試料均一性の増大や鉛濃度の濃縮によって、これまで鉛低濃度により測定が制約されていた生物試料のXANESスペクトルの臓器別、内容物別の測定に成功した。野生ラット3個体を主として、臓器は心臓、脳、肺、脾臓、肝臓、腎臓、筋肉など、胃・小腸・大腸の内容物、および糞便に対して、スペクトルの質を考慮したうえで系統的な解析を試みた。鉛化学形態の参照用XANESとして、グルタチオンと鉛の結合体や経口曝露源と考えられる現地の鉛汚染土壌等のデータを用いた。スペクトル形状および参照スペクトルとの線形結合フィッティングの結果、臓器別の鉛形態の違いや、消化器系や体内へ吸収された際の鉛化学形態の変化に関する知見を得ることができた。トカゲ亜目(Trachylepis wahlbergii)計112匹の臓器（肝臓、肺、卵巣/精巣）、血液、胃内容物を採集し肝臓中の金属濃度の測定を行った。土壌、トカゲ臓器、生体内で鉛と結合することが知られているグルタチオンなどと鉛の結合試料のPb L3端 XANESスペクトルによる解析を行った。鉛の周囲にグルタチオンをはじめ硫黄化合物が存在することが示唆された。このため、硫黄K端のエネルギー領域も計測し、個体間及び臓器間で異なるスペクトルパターンを得た。化学形態に加えて多角的なデータの取得を目的に遺伝子解析も実施しデータベース上にない全ゲノム配列データを得た。肝臓、肺、卵巣ついてRNA-seqも実施した。関係省庁・現地獣医師の協力の下、ニワトリ（10個体）、イヌ（26個体）の死亡個体の肝臓、腎臓、骨、腸管、脳、心臓、脾臓を採材し、鉛など10元素の濃度測定も行った。ラットの動物実験による確認実験も実施している。
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
予定通り採材、解析を実施しており、成果発表も充分に実施しているため。
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| Strategy for Future Research Activity |
今後は、今年度に引き続きXAFS解析、LA-ICP-MS解析などを行う予定である。遺伝子解析やエピジェネティクス解析も同じ個体から行うことで、遺伝子変異以外の金属耐性機構のメカニズム解明を行っていく予定である。
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| Causes of Carryover |
コロナウイルス感染症により予定していた海外渡航およびこれに関わるサンプリングの謝金支出が大幅に減額されたため。次年度には予定通り海外渡航を行う予定である。
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[Presentation] Lead concentrations and isotope ratios in blood, breastmilk and feces: contribution of both lactation and soil/dust exposure to infants in a lead mining area, Kabwe, Zambia2021
Author(s)
Shouta M.M. Nakayama, Haruya Toyomaki, John Yabe, Yared Beyene Yohannes, Kaampwe Muzandu, Tiza Mufune, Hokuto Nakata, Yoshinori Ikenaka, Takeshi Kuritani, Mitsuhiro Nakagawa, Kennedy Choongo, Mayumi Ishizuka
Organizer
SOT2022
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[Presentation] Land Use in Habitats Affects Metal Concentrations in Wild Lizards Around a Former Lead Mining Site2021
Author(s)
Nakayama SMM, Doya R, Nakata H, Toyomaki H, Yabe J, Muzandu K, Yohannes Y, Kataba A, Zyambo G, Ogawa T, Uchida Y, Ikenaka Y, Ishizuka M
Organizer
SETAC Europe 31st Annual Meeting
Int'l Joint Research
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[Presentation] (Epi) Genetic Effects of δ-Aminolevulinic Acid Dehydratase (ALAD) in Children Exposed to Environmental Lead - a primary study from Kabwe, Zambia2021
Author(s)
Yared Beyene Yohannes, Shouta M.M. Nakayama, John Yabe, Hokuto Nakata, Haruya Toyomaki, Andrew Kataba, Kaampwe Muzandu, Kennedy Choongo, Yoshinori Ikenaka, Mayumi Ishizuka
Organizer
48th Annual meeting of Japanese Society of Toxicology
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[Presentation] Isotopic Evaluation of the Contribution of Dust from a Mining Site to Heavy Metal Exposure to Wild Lizards2021
Author(s)
Doya Rio, Nakayama M.M. Shouta, Nakata Hokuto, Toyomaki Haruya, Yabe John, Muzandu Kaampwe, Yohannes B. Yared, Kataba Andrew, Kuritani Takeshi, Nakagawa Mitsuhiro, Uchida Yoshitaka, Ikenaka Yoshinori, Ishizuka Mayumi,
Organizer
The 9th SaSSOH, 2021
Int'l Joint Research
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[Presentation] Eco-Toxicological Investigation into Lead Exposure to Wild Lizards via Respiratory System2021
Author(s)
Rio Doya, Shouta M.M. Nakayama, Hokuto Nakata, Haruya Toyomaki, John Yabe, Kaampwe Muzandu, Yared B. Yohannes, Andrew Kataba, Yoshitaka Uchida, Yoshinori Ikenaka, and Mayumi Ishizuka
Organizer
The 14th Asian Society of Conservation Medicine
Int'l Joint Research
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[Presentation] Wild birds lead poisoning in Honshu area2021
Author(s)
Kohei Ogasawara, Hikaru Adachi, Haruko Kono, Shouta M.M. Nakayama, Naoki Tomita, Chihiro Ishii, Yoshinori Ikenaka, Yasuko Iwami, Yukiko Watanabe, Manabu Onuma, Mayumi Ishizuka, Keisuke Saito
Organizer
The 14th Asian Society of Conservation Medicine
Int'l Joint Research
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[Presentation] Genetic Polymorphism and Promoter Methylation of δ-Aminolevulinic Acid Dehydratase (δ-ALAD) in Children from Kabwe, Zambia2021
Author(s)
Yared Beyene Yohannes, Shouta M.M. Nakayama, John Yabe, Hokuto Nakata, Haruya Toyomaki, Andrew Kataba, Kaampwe Muzandu, Kennedy Choongo, Yoshinori Ikenaka, Mayumi Ishizuka
Organizer
5th International Chemical Hazard Symposium
Int'l Joint Research
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[Presentation] The Impact of lead poisoning in Kabwe; a holistic approach and potential mitigation measures2021
Author(s)
Yabe J, Nakayama S, Nakata H, Yohannes YB, Toyomaki H, Muzandu K, Moonga G, Kataba A, Zyambo G, Munyinda NS, Mufune T, Liazambi A, Choongo K, Ikenaka Y, Ishizuka M
Organizer
International Symposium on Mine Impact on One Health
Int'l Joint Research
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[Presentation] Toxicity assessment of lead in Kabwe residents and its effect of maternal quality of life2021
Author(s)
Nakata H, Nakayama S, Yabe J, Kaampwe M, Tohyama H, Fujita W, Kataba A, Yohannes YB, Toyomaki H, Zyambo G, Munyinda N, Sakala D, Yamsaki S, Nagai N, Yoshida T, Saito T, Choongo K, Ikenaka Y, Ishizuka M
Organizer
International Symposium on Mine Impact on One Health
Int'l Joint Research
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[Presentation] EVALAUTION OF LEAD EXPOSURE IN COW MILK FOR RISK ASSESSMENT IN KABWE, ZAMBIA2021
Author(s)
Golden Zyambo, John Yabe, Kaampwe Muzandu, Ethel M’kandawire, Kennedy Choongo, Andrew Kataba, Kenneth Chawinga, Allan Liazambi, Shouta M. M. Nakayama, Hokuto Nakata, Mayumi Ishizuka
Organizer
International Symposium on Mine Impact on One Health
Int'l Joint Research
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[Presentation] Lead Exposure and its Epi- (Genetic) Effects on Children in Kabwe, Zambia2021
Author(s)
Yared Beyene Yohannes, Shouta M.M. Nakayama, John Yabe, Hokuto Nakata, Haruya Toyomaki, Andrew Kataba, Kaampwe Muzandu, Kennedy Choongo, Yoshinori Ikenaka, Mayumi Ishizuka
Organizer
International Symposium on Mine Impact on One Health
Int'l Joint Research
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[Presentation] Determination of heavy metals and trace elements in organs of stray dogs from Kabwe, Zambia2021
Author(s)
Nyein Chan Soe, Shouta M.M. Nakayama, John Yabe, Hokuto Nakata, Yared B. Yohannes, Kenneth Chawinga, Golden Zyambo, Kaampwe Muzandu, Andrew Kataba, Patricia Bwalya, Geoffrey Mainda, Yoshinori Ikenaka, Mayumi Ishizuka
Organizer
International Symposium on Mine Impact on One Health
Int'l Joint Research
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[Presentation] How land use patterns affect animal exposure to Pb in Kabwe?2021
Author(s)
Rio Doya, Shouta M.M. Nakayama, Hokuto Nakata, Haruya Toyomaki, John Yabe, Kaampwe Muzandu, Yared B. Yohannes, Andrew Kataba, Yoshitaka Uchida, Yoshinori Ikenaka, Mayumi Ishizuka
Organizer
International Symposium on Mine Impact on One Health
Int'l Joint Research
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[Presentation] Outline of the pilot-scale experiments at the University of Zambia2021
Author(s)
Mufalo, W., Tangviroon, P., Igarashi, T., Ito, M., Sato, T., Chirwa, M., Nyambe, I., Nakata, H., Nakayama, S., Ishizuka, M
Organizer
International Symposium on Mine Impact on One Health
Int'l Joint Research
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[Presentation] Bio-accessibility of potentially toxic elements from Kabwe mine wastes2021
Author(s)
Mufalo, W., Tangviroon, P., Igarashi, T., Ito, M., Sato, T., Chirwa, M., Nyambe, I., Nakata, H., Nakayama, S., Ishizuka, M
Organizer
International Symposium on Mine Impact on One Health
Int'l Joint Research
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[Presentation] Lead-bearing soil dispersion simulations in the Kabwe mine, Zambia2021
Author(s)
Shinsaku Nakamura, Toshifumi Igarashi, Yoshitaka Uchida, Mayumi Ito, Kazuyo Hirose, Tsutomu Sato, Walubita Mufalo, Meki Chirwa, Imasiku Nyambe, Hokuto Nakata, Shouta Nakayama, Mayumi Ishizuka
Organizer
International Symposium on Mine Impact on One Health
Int'l Joint Research
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