Development of two-dimensional LC/IRMS for organic solvents

2023 Fiscal Year Final Research Report

• Project/Area Number: 20K20639
• Research Category: Grant-in-Aid for Challenging Research (Pioneering)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 64:Environmental conservation measure and related fields
• Research Institution: Shibaura Institute of Technology (2023); Akita Prefectural University (2020-2022)
• Principal Investigator: Kawashima Hiroto 芝浦工業大学, システム理工学部, 教授 (60381331)
• Project Period (FY): 2020-07-30 – 2024-03-31
• Keywords: LC/IRMS / 安定同位体 / 炭素安定同位体 / 有機溶媒 / 食品

Outline of Final Research Achievements

In 2004, a liquid chromatograph/stable isotope ratio mass spectrometer (LC/IRMS) was developed and commercialized for the high-precision analysis of carbon stable isotope ratios of water-soluble components using a wet combustion method. The objective of this study was to develop a more innovative LC/IRMS instrument capable of utilizing organic solvents as eluents. The results suggest that LC/IRMS can separate and analyze multiple water-soluble vitamins by combining first and second dimensional columns. The availability of organic solvents will expand the utility of LC/IRMS across various fields and for analyzing complex components that have been challenging to analyze previously.

Free Research Field

環境科学

Academic Significance and Societal Importance of the Research Achievements

既存のLC/IRMSでは，δ13Cは二酸化炭素の状態で分析するため，炭素を含んだ有機溶媒は今まで全く使用できなかったが，2次元LC/IRMSを利用することで有機溶媒の使用が出来る可能性を示すことが出来た。一般的に，HPLCやLC/MSでは溶離液は水ではなく，有機溶媒の使用が想定されているため，多様なLCカラム（順相，逆相，イオン交換等）を選択できるようになり，疎水性の農薬類，核酸，アミノ酸，脂肪酸，ビタミン，医薬品等まで対象成分が飛躍的に広がることが予想される。