2022 Fiscal Year Final Research Report
Analysis of Protein Phosphorylation Networks with Designed Kinase and Proteomics
| Project/Area Number |
20K21272
|
|---|
| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Medium-sized Section 38:Agricultural chemistry and related fields
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2020-07-30 – 2023-03-31
|
| Keywords | 植物 / 遺伝子重複 / キナーゼ / CK1 |
|---|
| Outline of Final Research Achievements |
We have previously reported that the CKL (Casein Kinase 1-like) gene family is involved in circadian clock regulation in Arabidopsis thaliana, based on analyses using small molecule inhibitors and multiple gene knockdown. However, it is less known that CKL is involved in physiological phenomena other than the clock. The objective of this study was to identify the phosphorylation substrates by creating a modified CKL and performing proteomics of the plant expressing the modified CKL, and ultimately to clarify the physiological processes in which CKL is involved.
Although we ran into unexpected problems in the preparation of the materials, we were able to overcome some of them, and we expect to identify substrates using these materials.
|
| Free Research Field |
植物生理学, 農芸化学
|
| Academic Significance and Societal Importance of the Research Achievements |
植物の発生・生理・代謝などあらゆる生命現象の分子機構は, おもに変異体スクリーニングを起点とする遺伝学と原因遺伝子の解析から進められてきた. 順遺伝学の弱点として遺伝学的冗長性のある遺伝子を生命現象の鍵因子として発見しにくい. 現存する植物は染色体倍加の過程を経ており, 重複機能をもつ遺伝子が多く存在していると考えられる.
本研究は, プロテオミクス解析によってCK1の基質タンパク質およびCK1の関わる生理機能を網羅的に同定することを目指した. このようなアプローチは, 重複性の問題だけでなく, タンパク質翻訳後修飾を俯瞰する上で重要な取り組みとなろう.
|
