Establishment of a method to analyze RNA cleavage fragments by using tRNA ligase

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K21288
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 38:Agricultural chemistry and related fields
• Research Institution: Osaka Prefecture University
• Principal Investigator: Iwata Yuji 大阪府立大学, 生命環境科学研究科, 准教授 (80704965)
• Project Period (FY): 2020-07-30 – 2022-03-31
• Keywords: tRNAリガーゼ / 2',3'環状リン酸基

Outline of Final Research Achievements

This project aimed to clone RNA fragments with 2’,3’-cyclic phosphate at 3’ end generated by plant IRE1, an endoplasmic reticulum membrane-localized kinase/ribonuclease, using Arabidopsis tRNA ligase. By using recombinant Arabidopsis IRE1B, we cloned and sequenced IRE1B-cleaved several RNA fragments and identified features of RNA sequences targeted by IRE1B. We also generated transgenic Arabidopsis plants that can be used to identify target RNA sequences by IRE1 in vivo.

Free Research Field

植物分子生物学

Academic Significance and Societal Importance of the Research Achievements

本研究により得られた成果は、これまで未知だった植物IRE1によるRegulated IRE1 dependent decay（RIDD）の標的となる塩基配列の解明や、小胞体膜上におけるRNA分解制御の全容解明へ向けて大きな前進となる。