2022 Fiscal Year Final Research Report
Rapid and specific diagnosis for tumor using exosome and cfDNA
| Project/Area Number |
20K21375
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 42:Veterinary medical science, animal science, and related fields
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| Research Institution | Kagoshima University |
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Principal Investigator |
Miura Naoki 鹿児島大学, 農水産獣医学域獣医学系, 教授 (80508036)
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| Co-Investigator(Kenkyū-buntansha) |
高橋 雅 鹿児島大学, 農水産獣医学域獣医学系, 准教授 (40750419)
古澤 悠 鹿児島大学, 共同獣医学部, 特任助教 (30791793)
畑井 仁 岩手大学, 農学部, 特任教授 (40566535)
川原 幸一 大阪工業大学, 工学部, 教授 (10381170)
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| Project Period (FY) |
2020-07-30 – 2023-03-31
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| Keywords | 犬 / エクソソーム / non-coding RNA / cfDNA / 腫瘍 |
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| Outline of Final Research Achievements |
Tumor-specific exosome analysis revealed differences in surface lectin expression between cell lines, including decreased expression associated with canine mammary tumor metastasis. Exosome-containing RNA analysis identified specific changes in metastatic and primary canine melanoma cell lines. Clinical canine samples were used to determine the sensitivity and specificity of exosome-containing target microRNAs in case-control studies. In addition, we found specificity for the inclusion of non-coding RNAs other than microRNAs and reported the results. In tumor-specific cfDNA analysis, we selected SNPs in canine melanoma tissues by next-generation sequencing and confirmed mutations in four completely novel SNPs. Still, we could not confirm mutations in clinical samples.
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| Free Research Field |
臨床獣医学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では，獣医臨床領域では初めてとなるエクソソームの腫瘍特異的な表面レクチンの発現の違いを確認できた．この結果は，今後，腫瘍マーカのより選択的な選別の一助となると同時に，エクソソームを利用したDDSの標的ともなる．次にエクソソームに特異的に含有されるnon-coding RNAの網羅的解析から，腫瘍に特異的な発現含有パターンを確認することができ，今後の臨床バイオマーカーとして，より適切な指標が作成可能となる．特に転移病変と原発病変の細胞から分泌されるエクソソームの違いを確認できたことで，腫瘍のより選択的な病態の把握が可能となる．
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