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2022 Fiscal Year Final Research Report

Whole genome insertion of a giant virus into Chlorella and the mechanism of evolution of symbiosis

Research Project

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Project/Area Number 20K21452
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 45:Biology at organismal to population levels and anthropology, and related fields
Research InstitutionNagahama Institute of Bio-Science and Technology

Principal Investigator

Ogura Atsushi  長浜バイオ大学, バイオサイエンス学部, 教授 (60465929)

Co-Investigator(Kenkyū-buntansha) 保科 亮  長浜バイオ大学, バイオサイエンス学部, 助教 (40373089)
Project Period (FY) 2020-07-30 – 2023-03-31
Keywords巨大ウイルス / 藻類 / 遺伝子移行 / ゲノム
Outline of Final Research Achievements

In this study, to investigate the presence of giant viruses and their effect on the genomes, we performed genome assembly of several Chlorella species. Using a hybrid assembly approach combining short-read and long-read data, we successfully assembled the genomes of 3 species with high accuracy. We then focused on the understanding of genomic changes that occur during the early stages of secondary endosymbiosis in symbiotic organisms. We compared the genome of C. variabilis with closely related free-living species. We also analysed transcriptomes under symbiotic and nonsymbiotic conditions to elucidate genetic mechanisms underlying symbiosis. To verify the expression of giant virus genes in algal genomes, we also performed functional analyses. We identified differentially expressed genes. Our functional analysis revealed that while most of the giant virus-derived genes were not expressed, some genes showed confirmed expression.

Free Research Field

進化学

Academic Significance and Societal Importance of the Research Achievements

我々は、緑藻ゲノムにほぼ全長の巨大ウイルスが挿入されていることを発見した。真核生物のゲノム内における巨大ウイルスゲノムの発見は、ウイルス学、ゲノミクス、進化生物学など、生物学の複数の分野に大きな影響を与えることになる。ウイルスの進化とウイルス学の理解につながることが期待され、真核生物における巨大ウイルスのゲノムの発見は、ウイルスの進化や細胞生命との関わりについて、さらなる知見が得られる。また、新しいウイルスファミリーや新しいタイプのウイルスの発見にもつながる。真核生物と巨大なウイルスがゲノムレベルでどのように相互作用しているかについての知見が得られる。

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Published: 2024-01-30  

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