2022 Fiscal Year Final Research Report
Clarification of the origin and maintenance mechanisms of junctional epithelium and identification of its stem cells using allogenic tooth germ transplantation
| Project/Area Number |
20K21672
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 57:Oral science and related fields
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
常木 雅之 新潟大学, 歯学部, 研究支援者 (40714944)
依田 浩子 新潟大学, 医歯学系, 准教授 (60293213)
原田 英光 岩手医科大学, 歯学部, 教授 (70271210)
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| Project Period (FY) |
2020-07-30 – 2023-03-31
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| Keywords | 接合上皮 / 歯肉 / 遺伝子改変マウス / 歯の萌出 / 移植 / 体性幹細胞 / 退縮エナメル上皮 / 上皮付着 |
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| Outline of Final Research Achievements |
This study clearly demonstrated the fate of junctional epithelium derived from the reduced enamel epithelium. The junctional epithelium showed GFP-negative reaction when tooth germs from wild-type (WT) were transplanted into GFP-transgenic mice. In contrast, the junctional epithelium maintained GFP-positive reaction during the experimental periods when tooth germs from GFP-transgenic mice were transplanted into WT mice. In addition, basal cells of the junctional epithelium maintained high proliferative potential throughout the experimental periods. These observations indicate that the adult stem cell niche derived from the reduced enamel epithelium is maintained in the junctional epithelium for a long time without being replaced by the oral epithelium. Chase experiments suggest that junctional epithelial cells were replenished from proliferative stem/progenitor cells in the basal junctional epithelium and migrated to the oral side.
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| Free Research Field |
口腔解剖学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では，歯胚移植のための精緻なマウスモデルの構築に成功し，接合上皮における退縮エナメル上皮由来の細胞ニッチが歯の萌出後長期間維持されることを証明した．退縮エナメル上皮由来体性幹細胞の分離と細胞表面マーカーの解明は，接合上皮幹細胞の利用を可能にするものである．接合上皮は歯原性であることから，歯原性上皮の供給源として利用の可能性がある．接合上皮幹細胞と歯髄幹細胞，脱落乳歯幹細胞，歯根膜幹細胞，根尖歯髄幹細胞，歯小嚢細胞，歯肉由来間葉系幹細胞などの間葉系幹細胞を用いた歯のバイオエンジニアリングは，将来の歯科再生医療につながると思われる．
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