2021 Fiscal Year Final Research Report
Construction of optimization system for hard tissue regenerating cells based on transcription network analysis
| Project/Area Number |
20K21687
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 57:Oral science and related fields
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| Research Institution | Showa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
山田 篤 昭和大学, 歯学部, 講師 (50407558)
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| Project Period (FY) |
2020-07-30 – 2022-03-31
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| Keywords | 骨再生 |
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| Outline of Final Research Achievements |
A part of the neural crest-derived cells maintain their properties as stem cells even after growth and have pluripotency, so they are expected to be a new cell source for regenerative medicine. In this study, we use neural crest-derived cells present in transgenic mice (P0-Cre / GFP mice) that express GFP, and induce osteoblasts using neural crest-derived cells. The gene expression of osteoblasts derived from neural crest-derived cells will be analyzed in detail, and the value as a cell source applied to the induction of bone formation based on the results will be clarified. At that time, in addition to the conventional transcriptome analysis, a comprehensive analysis of transcriptional regulation was performed by identifying the open chromatin region.
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| Free Research Field |
骨代謝
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| Academic Significance and Societal Importance of the Research Achievements |
体性幹細胞である神経堤細胞を再生医療で用いる利点として腫瘍のリスクが低く、安全性が高い、多分化能という特性のみならず、臓器の障害や機能低下を修復改善する治療効果を有している場合が多く、成体の障害臓器に対する細胞治療として効率が良い、また、採取される細胞のバックグラウンドが明確であるため、標的とする組織に対する組織特異性を高めることができるなどがある一方、資源が有限であり、品質の維持、中でも均一な幹細胞の採取が困難であるなどの欠点が挙げられる。そうした、組織再生における問題点を克服するために、使用する細胞の特性、中でも遺伝子発現様式の網羅的解析は重要であると考えられる。
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