2022 Fiscal Year Final Research Report
Development of a rapid and easy method for evaluating human transmission risk based on an enzymatic activity of avian influenza virus
| Project/Area Number |
20K21721
|
|---|
| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Medium-sized Section 58:Society medicine, nursing, and related fields
|
|---|
| Research Institution | University of Shizuoka |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
池田 潔 広島国際大学, 薬学部, 教授 (40168125)
|
|---|
| Project Period (FY) |
2020-07-30 – 2023-03-31
|
| Keywords | シアリダーゼ / ノイラミニダーゼ / 酵素 / インフルエンザウイルス / 蛍光検出 |
|---|
| Outline of Final Research Achievements |
Cases of human infection with H7N9 avian influenza A virus have been reported in China since 2013. To elucidate the function of the viral surface glycoprotein neuraminidase (NA) associated with different host transmission from birds to humans, we investigated the enzymatic properties of NA in H7N9 avian virus isolated from humans in 2013. The properties of H7N9 avian virus NA were similar to those of most avian virus NAs and were different from those of most human virus NAs. Therefore, we identified amino acid changes in N9NA responsible for the pH-dependent enzymatic property that is significantly different between avian and human virus NAs, and demonstrated that this enzymatic property has a significant effect on virus growth.
|
| Free Research Field |
糖鎖生物学
|
| Academic Significance and Societal Importance of the Research Achievements |
2013年にヒト感染例が多く報告された鳥インフルエンザウイルスのN9型NAの性状は、一般的な鳥ウイルスとほぼ同様であった。NAの性状の観点から、ヒト感染に関わる要因を明らかにできなかった。しかし、そのN9型NAの1系統は、中性における活性が低下していた。これは、一般的なウイルスNAの性状ではなかった。季節性ヒトウイルスNAに見られる酸性に不安定なNAや中性における活性が低下したNAは、N9型NAにおいてウイルス増殖を低下させた。これらのNAの性状は、ウイルス増殖性に大きく関わっていた。
|
