2021 Fiscal Year Final Research Report
Individual fluid sampling and isolation of bacteria in fluid inclusion
| Project/Area Number |
20K21839
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 63:Environmental analyses and evaluation and related fields
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
平野 伸夫 東北大学, 環境科学研究科, 助教 (80344688)
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| Project Period (FY) |
2020-07-30 – 2022-03-31
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| Keywords | 地下生物圏 / 流体包有物 / 海底噴気堆積鉱床 / 微生物隔離 / ラマン分光計測 |
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| Outline of Final Research Achievements |
We succeeded in synthesizing fluid inclusions artificially by encapsulating albumin solution in quartz, and then conducted Raman spectroscopy of these fluid inclusions to obtain spectra derived from C-H bonds. Based on these results, we attempted Raman spectroscopy of fluid inclusions in black ore samples from a natural black ore deposit (Ainai Mine, Akita Prefecture), and obtained spectra derived from C-H bonds similar to those of albumin, indicating that the black ore contains organic matter. Although we have not yet obtained proof of the presence of microorganisms, we believe that this method can be applied to the search for the presence of microorganisms associated with marine hydrothermal sedimentary deposits (VMS: Volcano hosted Massive Sulfides) and the underground biosphere.
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| Free Research Field |
地圏環境科学
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| Academic Significance and Societal Importance of the Research Achievements |
地圏環境にはまだ未発見の生物が隠されており，それらは岩石・鉱石中の流体包有物中に隔離されていると推定される．この生物痕を調査するために，流体包有物を個別にラマン計測する方法を考案し，海底噴気堆積鉱床の黒鉱中の流体包有物に有機物が含まれることをはじめて認めた．まだ生物であるかどうかの確証は得られていないが，この方法を展開することにより未知の生物の探索につながると期待される．
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