2022 Fiscal Year Final Research Report
Nano-Scale observation of the decomposition in bacteria inhabit environment toward for sustainable development of fisheries industry
Project/Area Number |
20K21854
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Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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Allocation Type | Multi-year Fund |
Review Section |
Medium-sized Section 64:Environmental conservation measure and related fields
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Research Institution | Doshisha University (2021-2022) Hirosaki University (2020) |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
井上 晴彦 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 上級研究員 (10435612)
塚本 勝男 大阪大学, 大学院工学研究科, 招へい教授 (60125614)
桐原 慎二 弘前大学, 地域戦略研究所, 教授 (60519594)
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Project Period (FY) |
2020-07-30 – 2023-03-31
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Keywords | 細菌 / 溶解 / 結晶表面 |
Outline of Final Research Achievements |
The cleavage surface of the calcite was exposed to the culture fluid of the bacteria for 4 days to investigate the effect of bacteria on the calcite dissolution kinetics. The surface morphology of the calcite was investigated using an optical microscope and scanning electron microscope after the dissolution experiment in the culture fluid with bacteria. The calcium ion concentration in the culture fluid of Streptomyces was one-third of control, Escherichia coli DH5alpha. Meanwhile, the calcite surface, which has been exposed in the culture fluid of bacteria shows the etch pits, which were formed during the dissolution process. These etch pits were formed accompanied by the bacteria colony, which were formed along the cleavage step on the calcite surface. These observations infer that the inhabitation of the bacteria enhance the dissolution of the calcite and also promoted the precipitation of calcium ion. The coupling of these effects promoted the dissolution process of calcite.
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Free Research Field |
結晶成長
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Academic Significance and Societal Importance of the Research Achievements |
養殖などで大量に発生する貝殻を生物活動で安価に分解する技術は、わが国の水産業の持続的発展にとって必要な技術である。特殊な細菌が自然環境において無機結晶の成長を促進することは良く知られており、様々な研究が行われてきたが、どのように生物活動により溶解が促進されるのかそのメカニズムには不明な点が多かった。今回、細菌による方解石の分解プロセスを詳細に観察することによって細菌の方解石に対する分解メカニズムの一端が明らかになった。この結果は貝殻処理の低コスト化につながるだけでなく、石灰岩が分解されアルカリ土壌が形成されるメカニズムについても、新しい知見を得た。
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