2022 Fiscal Year Final Research Report
Development of novel microstructure culture substrate using in-vivo matrix processing method
Project/Area Number |
20K22489
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Multi-year Fund |
Review Section |
0403:Biomedical engineering and related fields
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Research Institution | Asahikawa Medical College |
Principal Investigator |
SATO Yasushi 旭川医科大学, 医学部, 助教 (30767778)
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Project Period (FY) |
2020-09-11 – 2023-03-31
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Keywords | 組織工学 / 微細培養基板 / 臓器チップ / in vitro評価系 / 生体内組織工学 |
Outline of Final Research Achievements |
The aim of this study was to develop biological tissues with controlled shape and apply them to culture plates for in vitro evaluation system that enables more accurate evaluation than conventional methods. The molds designed for the culture plate were embedded subcutaneously into the dorsal skin of rats, resulting in tissues formation. Depending on the molds design, it was possible to fabricate tissues with three-dimensional shapes, such as slit structures and multi-well structures, which could be applied as culture plates. When the cultured cells were seeded onto the fabricated tissues, it was observed that the cells adhered with similar morphology and density as those on normal cell culture plates. Additionally, cell proliferation occurred with the same efficiency as that observed in normal cell culture plates. These results suggest that tissues fabricated subcutaneously may be applicable as culture plates.
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Free Research Field |
細胞組織工学
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Academic Significance and Societal Importance of the Research Achievements |
本研究では、設計した鋳型を皮下に植え込むことにより、任意形状の生体組織体が作製可能であり、さらにそれを培養基板として応用できる可能性が示された。生体内と同じECM構造・密度を有する培養基板であるため細胞―ECM間相互作用が活性化され、従来の二次元培養で問題となる細胞機能低下を防ぎ、細胞の品質を維持したまま培養できる可能性がある。そのため、従来よりも高精度なin vitro評価系が構築でき、医薬品等の開発に応用されることが期待される。
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