Development of a fluorescent probe which enables organlle-selective imaging of intracellular behaviors of magnesium ion

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K22536
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0501:Physical chemistry, functional solid state chemistry, organic chemistry, polymers, organic materials, biomolecular chemistry, and related fields
• Research Institution: Keio University
• Principal Investigator: Sakama Akihiro 慶應義塾大学, 理工学部(矢上), 研究員 (40878170)
• Project Period (FY): 2020-09-11 – 2023-03-31
• Keywords: 機能性蛍光プローブ / 細胞イメージング / マグネシウムイオン

Outline of Final Research Achievements

A novel near-infrared magnesium ion (Mg2+) probe with HaloTag ligand to visualize organelle-selective Mg2+ dynamics in cells and its correlation with Mg2+-related signals by multicolor imaging. The probe localized to mitochondria and other organelles with or without HaloTag formation. Since this phenomenon was presumably due to the high hydrophobicity of the probe, two probes employing more hydrophilic ligands were synthesized. Although these probes were hardly Mg2+-responsive, it was suggested that they can be applied as low-affinity calcium ion probes.

Free Research Field

有機合成化学

Academic Significance and Societal Importance of the Research Achievements

HaloTagタンパク質リガンドを有する非対称Si-ローダミン型マグネシウム（Mg2+）プローブの合成法を確立したことにより、細胞内Mg2+と関連シグナルのマルチカラーイメージング技術の確立やその技術を用いた新たな生命現象の発見に向けた研究、および他のタグタンパク質リガンドを導入したSi-ローダミン型プローブの開発に貢献することが期待される。また、併せて開発した低親和性カルシウムイオンプローブは、従来の高親和性プローブでは可視化できなかった生命現象の発見やメカニズム解明に寄与することが期待される。