Physiological analysis of the resistant mechanisms of soybean for Spodotera litura

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K22584
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0602:Agricultural and environmental biology and related fields
• Research Institution: Kyoto University of Advanced Science
• Principal Investigator: Nakata Ryu 京都先端科学大学, バイオ環境学部, 講師 (90882548)
• Project Period (FY): 2020-09-11 – 2022-03-31
• Keywords: ダイズ / ハスモンヨトウ / 抵抗性 / 作用機構 / プロテアーゼインヒビター

Outline of Final Research Achievements

In order to elucidate the resistant mechanisms of soybean for Spodoptera litura, the physiological changes within the body of the larvae of S. litura fed on the soybean variety "Himeshirazu" were clarified, and the factors that cause these changes were investigated. Focusing on digestive enzyme activity in the midgut, we measured enzyme activity after accurately measuring pH in the midgut, and found a decrease in protease activity. Therefore, we examined the appropriate conditions for extracting protease inhibitors from the leaves of Himeshirazu. Then, we proceeded with purification using protease inhibitory activity as an indicator, and found a component characteristic of Himeshirazu.

Free Research Field

化学生態学

Academic Significance and Societal Importance of the Research Achievements

これまでの、ダイズの抵抗性遺伝子の特定が重視され抵抗性の作用機構に関する研究はあまり進んでいない。本研究ではハスモンヨトウ幼虫の生理的な変化に着目し、抵抗性の作用機構の解明を目指した。本研究成果により、ダイズ品種「ヒメシラズ」を摂食したハスモンヨトウ幼虫において中腸内のプロテアーゼ活性が低下することが明らかになり、その原因物質(阻害剤)がヒメシラズに含まれることが明らかになった。この成果は、抵抗性の作用機構の理解に基づいた有用な強抵抗性品種の開発に繋がると期待される。