Analyses of post-transcriptional regulation of RNA by in situ sequencing

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K22654
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0702:Biology at cellular to organismal levels, and related fields
• Research Institution: Kyoto University
• Principal Investigator: Sunadome Kazunori 京都大学, 高等研究院, 特定助教 (60872992)
• Project Period (FY): 2020-09-11 – 2022-03-31
• Keywords: 空間トランスクリプトミクス / 転写後制御 / スプライシング

Outline of Final Research Achievements

In this study, RNA localization pattern in muscle cells from human and mouse was examined with hybridization-based in situ sequencing (HybISS). The results revealed the gent-to-gene and species-to-species diversity of RNA localization pattern. Particularly, transcripts in human tend not to be exported after splicing compared to mouse. Also, nuclear export of these RNA molecules was enhanced with imposed static tension. These results suggest the relation among RNA splicing, localization, and static tension, which could produce differences in cellular system of species.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

本研究では、タンパク質の設計図であるRNAが細胞内のどこにどのタイミングで配置されるか、筋細胞をモデルにヒトとマウスの違いについて空間トランスクリプトミクス技術を用いて解析し、遺伝子間、種間のRNA局在パターンの多様性を明らかにした。ヒトと他の哺乳類の細胞システムの違いを生み出す要因について未だ多くの事が分かっておらず、本研究を元に種の特異性を制御する、遺伝子の転写後調節システムの解明が進む事が期待される。