2023 Fiscal Year Final Research Report
Investigation of the mechanism governing malaria parasite erythrocyte invasion regulated by DGK
| Project/Area Number |
20K22782
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0803:Pathology, infection/immunology, and related fields
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| Research Institution | Rakuno Gakuen University (2023)
Nagasaki University (2020) |
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Principal Investigator |
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| Project Period (FY) |
2023-02-26 – 2024-03-31
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| Keywords | ネズミマラリア原虫 / 赤血球侵入 / 脂質シグナリング / 遺伝子編集 |
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| Outline of Final Research Achievements |
The functional analysis of diacylglycerol kinase (DGK), responsible for the conversion of diacylglycerol (DAG) to phosphatidic acid (PA) in malaria parasite, elucidated that the inducible DGK deletion mutant exhibited the phenotype characterized by mislocalization of invasion related proteins, reduction of protein secretion, and subsequently decreased parasitemia and pathogenicity in mice.
The functional assessment of the APH protein which interacts with PA, a derivative of DGK1, disclosed that the inducible APH knockout parasite mirrored the inhibition of erythrocyte invasion related protein discharge as same as DGK1 deficient parasites.
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| Free Research Field |
寄生虫学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究課題を通してマラリア原虫が保有するDGKとPA結合タンパク質APHが原虫の赤血球侵入時に担う役割を分子レベルで解明することに成功した。また本研究で作製したDGK1欠損原虫は感染宿主内で自然治癒されるため、今後宿主応答の分子機序を精査することで新たな原虫制御法の確立に繋がる知見が得られることが期待できる。
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