2021 Fiscal Year Final Research Report
Analysis of the effect of cytokine production by autoantigen-reactive B cells in systemic sclerosis on other immune systems.
Project/Area Number |
20K22925
|
Research Category |
Grant-in-Aid for Research Activity Start-up
|
Allocation Type | Multi-year Fund |
Review Section |
0903:Organ-based internal medicine and related fields
|
Research Institution | The University of Tokyo |
Principal Investigator |
|
Project Period (FY) |
2020-09-11 – 2022-03-31
|
Keywords | 自己反応性B細胞 / サイトカイン / 全身性強皮症 / T細胞 / microfluidics / 単一細胞解析 |
Outline of Final Research Achievements |
In this study, we used original microspace-based techniques and methodologies to elucidate the function and role of autoreactive B cells in systemic sclerosis (SSc). Regarding the relationship between affinity to topoI antigen and cytokine production capacity, the proportion of B cells producing inflammatory cytokines such as IL-6 and IL-23 was higher in high affinity B cells, and the amount produced was also higher. Similarly, low affinity B cells produced a higher percentage of B cells that produced IL-10 and IL-35 inhibitory cytokines, and the amount produced was also higher. The former was also shown to induce Th17 cells and the latter Treg cells.
|
Free Research Field |
皮膚科学
|
Academic Significance and Societal Importance of the Research Achievements |
本研究は、自己抗原反応性B細胞の機能を明らかとすることで、数あるB細胞集団中から、病原性を有するB細胞集団を明らかとする。さらに我々の技術では、少数の自己抗原反応性B細胞のタンパク分析を可能としており、表面抗原の分析によって新たな治療ターゲットの同定につながることが期待される。このような研究は他に類が無く、他の自己免疫疾患においても応用可能であることから、革新的かつ創造的な研究となることが期待される。
|