Development of preventive treatment method by creating focal intestinal perforation rat model associated with prematurity aiming at improvement of neurological prognosis

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K22958
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0905:Surgery of the organs maintaining homeostasis and related fields
• Research Institution: Kagoshima University
• Principal Investigator: SUGITA Koshiro 鹿児島大学, 鹿児島大学病院, 特任助教 (50781514)
• Project Period (FY): 2020-09-11 – 2022-03-31
• Keywords: 限局性腸穿孔 / FIP / 新生児消化管穿孔 / 動物モデル

Outline of Final Research Achievements

Based on the hypothesis that the mechanism that causes diving reflex is the two stimulations of "infection" and "hypoxia" conducted in this study, an animal model was created by two-step stress stimulation, "maternal" and "postnatal" was verified.
The purpose was to reproduce the diving reflex and FIP model by performing it on pregnant rats, but no results were obtained to develop FIP. Human stress hormones are endogenous steroids that are favored in the adrenal glands, but prenatal fetal stress hormones are said to be vasopressin, which has a stronger vasoconstrictor effect. We will continue to explore the selection of reagents and stimuli for stress during pregnancy in order to reproduce the diving reflex, and we believe that further research is needed to create an FIP model.

Free Research Field

小児外科

Academic Significance and Societal Importance of the Research Achievements

FIPは超低出生体重児に発症するが、希少疾患であるため臨床情報からだけではエビデンスが得られにくい。2年間の本研究期間中に低酸素刺激とLPS刺激だけではFIPモデルを作成することはできなかった。作成を試みた結果、ＮＥＣとの病態的な違いにより一層着目していく必要を認識した。FIPはNECと異なり典型的には回盲部領域に発生する、回盲部領域の血流を評価することや血流が低下する原因として胎児ストレスホルモンの関連性などを明らかにしていく着想に至り、今後の研究課題を得られたという意味では有意義であったと思われた
。