The mechanisms of occlusal trauma focusing on mechanosensing by osteocyte

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K23020
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0907:Oral science and related fields
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Tsuchiya Yosuke 東京医科歯科大学, 歯学部附属病院, 医員 (40882072)
• Project Period (FY): 2020-09-11 – 2022-03-31
• Keywords: 咬合性外傷 / 骨細胞 / 歯根膜細胞 / メカニカルストレス

Outline of Final Research Achievements

Mice were divided into the following four groups: periodontitis (Pe) group, occlusal trauma+periodontitis(Pe+OT) group, occlusal trauma (OT) group and a control (Co) group.Pe+OT group showed significantly more bone loss than that of the Pe group at 8days after treatment.In addition, OT group showed more bone loss than that of Co group at 8days after treatment. These data suggesting that occlusal trauma is an aggravating factor for periodontitis and that occlusal trauma contributes to bone resorption in the mouse experiment.In addition, gingiva and alveolar bone samples were collected from the above four groups 3 days after each treatment, and comprehensive analysis using RNA-seq revealed that many genes with altered expression were found in the Pe+OT groups compared to the Pe group.

Free Research Field

歯周病学

Academic Significance and Societal Importance of the Research Achievements

咬合性外傷はかねてから歯周病の増悪に寄与する共同破壊因子として示されているものの、動物実験や臨床における症例観察研究、介入研究による根拠がほとんどでありその作用機序を分子生物学的に検討しようとした研究は乏しい。咬合性外傷がどの様なメカニズムで発生するか、その病因について解明することは臨床的な歯周病の治療戦略上有意義であり、歯周治療における咬合治療の必要性を裏付ける重要なエビデンスになりうる。今後さらなる解析を行いメカニズムの同定を目指す。