2021 Fiscal Year Final Research Report
Effect of oxygen concentration on glucose metabolism of cancer cells
| Project/Area Number |
20K23042
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0907:Oral science and related fields
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2020-09-11 – 2022-03-31
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| Keywords | 低酸素 / 糖代謝 / pHスタッドシステム / リアルタイムモニタリング / 口腔扁平上皮癌 |
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| Outline of Final Research Achievements |
Cancer cells cultured in hypoxia showed a higher metabolic activity in normoxic than hypoxic conditions (p<0.05). There were no significant differences between normoxic and hypoxic metabolic activity of normoxia-cultured cancer cells or hypoxia-cultured normal cells. The cancer cells cultured in hypoxia produced lactic acid in hypoxic conditions; however, they did not increase the lactic acid production in normoxic conditions, irrespective of their high metabolic activity. ROS production was higher in normoxic conditions in all cells, especially the hypoxically cultured HSC-3 cells.
Rapid increases in oxygen levels might enhance the glucose metabolism of hypoxically cultured cancer cells by mainly activating the TCA cycle and electron transport system. The activation of the TCA cycle and the electron transport system might increase the ATP and ROS supply in cancer cells through the oxidative phosphorylation.
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| Free Research Field |
歯科補綴学分野
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| Academic Significance and Societal Importance of the Research Achievements |
本研究において、細胞の代謝活性を低酸素環境においてリアルタイムにモニタリングすることで、がん周囲環境中の酸素濃度の変動が、がん細胞の代謝に影響を及ぼすこと、さらには単純な酸素濃度の違いだけはなく、高酸素から低酸素、低酸素から高酸素濃度条件といった濃度変動により、より多彩な代謝反応の変化が起きることが明らかとなった。本研究結果は、がん細胞の代謝は、複雑にその様相が変化していくがん周囲の微小環境を再現しながら探っていく必要性が高いこと、その際、単純に環境を変えるだけではなく、その変動という視点を加味することが必要であることを示唆し、これからの細胞代謝研究に新たな視点を与えるものとなった。
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