Elucidation of bone regeneration mechanism of SHED-CM on bone regeneration and clinical application for cleft palate bone regeneration treatment

2022 Fiscal Year Final Research Report

• Project/Area Number: 20K23055
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0907:Oral science and related fields
• Research Institution: Hiroshima University
• Principal Investigator: HIRAKI TOMOKA 広島大学, 医系科学研究科(歯), 研究員 (70881275)
• Project Period (FY): 2020-09-11 – 2023-03-31
• Keywords: SHED / 間葉系幹細胞 / 骨再生 / 培養上清

Outline of Final Research Achievements

We focused on stem cells from human exfoliated deciduous teeth (SHED) and condition media of SHED (SHED-CM). The purpose of this study is to elucidate the useful humoral factors for bone regeneration in SHED-CM for the treatment of jaw cleft bone regeneration.
In the first year, we clarified that bone regeneration was promoted by transplanting SHED-CM into a bone defect created in the mouse skull using a transplant carrier (atelocollagen). From the next year , MSCs-CM and SHED-CM were added to HUVEC, MC3T3-E1, and hBMSCs. We examined the expression levels of cell proliferation, bone morphogenetic factors and angiogenesis markers.

Free Research Field

骨再生

Academic Significance and Societal Importance of the Research Achievements

近年、間葉系幹細胞の培養上清について着目されているものの、その作用機序については不明な点が多い。本研究では、SHED-CMに含有される液性成分について解析し、移植を行うことで良好な骨再生を生じることを明らかとした。
低侵襲で良好な骨再生療法を確立することで、患者の負担を軽減できることは、社会的観点から重要な意義がある。さらに、SHED-CMの骨再生機構の解明を試みることで、歯科医学にとどまらず広く再生医療分野の進歩へ貢献を果たすと考えられる。