Development of evaluation of nutritional status dualing fetal and early postnatal using dentin analysis of primary teeth

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K23068
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0907:Oral science and related fields
• Research Institution: Fukuoka Dental College
• Principal Investigator: OHKI SHIRABE 福岡歯科大学, 口腔歯学部, 助教 (60878255)
• Project Period (FY): 2020-09-11 – 2022-03-31
• Keywords: 低栄養 / 乳歯 / DOHad説

Outline of Final Research Achievements

The expression of Tenascin C in normal mouse tooth germs from 13.5 days of embryonic age to 32 days of age, which is the stage of tooth germ formation, was observed by immunohistochemical staining. From embryonic 13.5 days to embryonic 15.5 days, Tenascin C was strongly expressed in the dental papilla and odontoblast layer. Expression was weak after 15.5 days of embryonic development. At 10 days of age, expression of Tenascin C was observed in the pre-dentin, but no clear expression was observed in the calcified dentin. In the dentin of human shed deciduous teeth, the expression of Tenascin C as observed in mice was not observed, but the expression along the dentin tubules was observed.

Free Research Field

小児歯科学

Academic Significance and Societal Importance of the Research Achievements

胎児期および出生後早期の低栄養環境が、成人期・老年期における生活習慣病のリスク要因となり得るというDOHaD説は、ヒトを対象とした出生コホート研究により実証されてきた。これまでの栄養評価は、主に出生時体重によってなされてきたが、出生時低体重の要因は早産、多胎、母体喫煙など様々であり、栄養状態の評価として体重だけでは不十分である。近年盛んに行われているゲノム解析に加え、過去の栄養状態を現時点で知るツールがあれば、DOHaD説の検証研究は飛躍的に進歩する。本研究により、胎児期、小児期に将来の健康寿命を予測できるようになれば、成人病予防を推進することに大きく寄与すると考えられる。