Development of in vitro analysis system for enamel calcification

2021 Fiscal Year Final Research Report

• Project/Area Number: 20K23070
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0907:Oral science and related fields
• Research Institution: Tohoku University
• Principal Investigator: Tadano Manami 東北大学, 大学病院, 医員 (00885535)
• Project Period (FY): 2020-09-11 – 2022-03-31
• Keywords: エナメル芽細胞 / 歯 / 三次元培養

Outline of Final Research Achievements

Ameloblast play a crucial role in tooth development, and enamel formation results from cell proliferation and differentiation through continuous epithelial-mesenchymal cell interactions. It is suggested that three-dimensional culture, in which cell-cell adhesion is closer than that in normal planar culture, affects differentiation. Therefore, we aimed to create a model for the evaluation of ameloblast differentiation and calcification, using rat-derived dental epithelial cell line SF2 cells in three-dimensional culture. As a result of the experiment, it was found that the secretion of enamel matrices was increased in the three-dimensional culture. Therefore, it was suggested that this culture method has been shown to be effective for efficient ameloblast differentiation.

Free Research Field

小児歯科

Academic Significance and Societal Importance of the Research Achievements

本研究を通じ、三次元培養下におけるラット由来歯原性上皮細胞株SF2細胞の培養によって、エナメル質に特異的な基質の誘導が生じることが明らかとなった。これらより新たな三次元培養技術と新規分子の応用によって、より効率的なエナメル芽細胞の誘導法の新技術開発に貢献することが期待される。