Investigation of Toxic Cyanobacteria in North Thailand

2011 Fiscal Year Final Research Report

• Project/Area Number: 21040412
• Allocation Type: Single-year Grants
• Research Institution: Nagasaki University
• Principal Investigator: ITAYAMA Tomoki 長崎大学, 大学院・工学研究科, 教授 (80353530)
• Co-Investigator(Kenkyū-buntansha): IWAMI Norio 明星大学, 理工学部, 准教授 (00353532) ; SUGIURA Norio 筑波大学, 生命環境科学研究科, 教授 (10302374) ; SIMIZU Kazuya 東洋大学, 生命科学部, 助教 (10581613)
• Co-Investigator(Renkei-kenkyūsha): SANO Tomoharu (独)国立環境研究所, 環境計測研究センター, 主任研究員 (10178808) ; PARK Hodong 筑波大学, 生命環境科学研究科, 教授 (20262686)
• Research Collaborator: WHANGCHAI Niwoot Maejo University, Thailand, Associate Professor ; PRAPHRUTE Ruenkeaw Maejo University, Technical Staff ; KANNIKA Korntip Phayao University, Thailand, Assistant Professor ; PROMMANA Rattapoom Phayao University, Thailand, Associate Professor ; GUTIERREZ Redel Central Luzon State University, Phillipine, Associate Professor ; PEERAPORNPISA Yuwadee Chiang Mai University, Thailand, Associate Professor
• Project Period (FY): 2009 – 2011
• Keywords: タイ / 養魚池 / 藍藻 / 藍藻毒microcysti / mcy遺伝子 / 定量PCR / microcystin分解菌

Research Abstract

We found toxic cyanobacteria such as Microcystis sp. in almost investigated fish ponds(including the experimental fish ponds of Maejo University) in North Thailand. Especially, the amount of toxin producing cyanobacteria in the catfish pond was much than that in the tilapia ponds. However we also found the high ratio of non-toxin-producing strains of Microcystis sp. in such fish ponds by a cyanotoxin microcystin analysis and qPCR for the microcystin synthetase mcy genes. A few amount of microcystin was detected from several fishes in the experimental ponds in Maejo University. On the other hand, musty odors substances produced by cyanobacteria were detected in many fishes and in many ponds. Therefore, because we thought that it is important to clarify such fish ponds using a bio-fence type biological filtration method, we conducted a preliminary experiment and mathematical analysis of purification process of the ponds. As a result, we presented that the bio-fence method should be developed as an adequate eco-engineering for safe fish production in aquaculture.

Research Products

• [Journal Article] How microcystin-degrading bacteria express microcystin degradation activity (2011)
  • Author(s): Kazuya Shimizu, Hideaki Maseda, Kunihiro Okano, Tomoaki Itayama, Yukio Kawauchi, Rongzhi Chen, Motoo Utsumi, Zhenya Zhang, Norio Sugiura
  • Journal Title: Lakes & Reservoirs : Research & Management Volume: 16巻 Pages: 169-178
  • Peer Reviewed
• [Journal Article] Norio Iwami and Tomoaki Itayama, Musty Odor in Tilapia(Oreochromis niloticus) Cultured in Cages and Earthen Ponds (2010)
  • Author(s): Niwooti Whangchai, Chayarat Pleumsumran, Siraprapa Fakrajang
  • Journal Title: Journal of Agricultural Research and Extension Volume: 27巻 Pages: 19-27
  • Peer Reviewed
• [Presentation] Water Purification by Bio-fence system (2011)
  • Author(s): Tomoaki Itayama, Norio Iwami, Xue-Ying Bi, Dong Xia, Kazuya Shimizu, Korntip Kannika, Redel Gutierrez, Fumiaki Furusawa, Kohmsan Ruangdet, Niwooti Whangchai and Norio Sugiura
  • Organizer: The fourth International Fisheries Conference Climate change : Impact on Aquatic Resources and Fisheries
  • Place of Presentation: Chiang Mai, Thailand
  • Year and Date: 2011-12-01
• [Presentation] Development of Controlling Methods of Toxic Cyanobacteria in Eutrophicated Reservoirs (2009)
  • Author(s): Tomoaki ITAYAMA, Nobuyuki TANAKA, Norio IWMAI, Takashi KUWABAAR, Niwooti WHANGCHAI
  • Organizer: World City Water Forum
  • Place of Presentation: Incheon, Korea(招待講演)
  • Year and Date: 20090818-21