Analysis of the mechanisms of the synapse formation of newborn neurons by developing mice with channelrhodopsin-2 only in newborn neuron in the dentate gyrus.

2011 Fiscal Year Final Research Report

• Project/Area Number: 21200016
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
• Allocation Type: Single-year Grants
• Research Field: Neurophysiology and muscle physiology; Psychiatric science
• Research Institution: Gunma University
• Principal Investigator: YASUDA Hiroki 群馬大学, 大学院・医学系研究科, 准教授 (60294071)
• Co-Investigator(Renkei-kenkyūsha): KANEKO Ryosuke 群馬大学, 大学院・医学系研究科, 助教 (40390695)
• Project Period (FY): 2009 – 2011
• Keywords: 神経新生 / 海馬歯状回 / チャネルロドプシン-2 / トランスジェニックマウス

Research Abstract

We planned to develop a new genetically modified mice whose newborn neurons in the dentate gyrus in the hippocampus posess channelrhodopsin-2 (chr2) to analyze the mechanisms of synapse formation of newborn neurons. We are still in the middle of generating the chr2 mice we originally planned, instead we used another mice in which neurons with markers of inhibitory cells express channelrhodopsin-2, because newborn excitatory neurons in the dentate gyrus also have these markers until about three weeks after birth. We now check whether light stimulation on the dentate gyrus evoked EPSCs in pyramidal neurons in the CA3 region and inhibitory neurons in the hilus.
Recent literature suggests that antidepressants improve depressed mood through increasing generation of newborn neurons in the dentate gyrus in the hippocampus, therefore we examined the effects of stress, which can cause depression, on the excitability of granule cells in the dentate gyrus. We found that forced swimming made granule neurons more excitable and now investigating the mechanisms that underlie the elevated excitability.

Research Products

• [Journal Article] Selective control of inhibitory synapse development by Slitrk3-PTPδ trans-synaptic interaction (2012)
  • Author(s): Hideto Takahashi, Kei-ichi Katayama, Kazuhiro Sohya, Hiroyuki Miyamoto, Tuhina Prasad, Yoshifumi Matsumoto, Maya Ota, Hiroki Yasuda, Tadaharu Tsumoto, Jun Aruga & Ann Marie Craig
  • Journal Title: Nature Neuroscience Volume: 15(3) Pages: 389-398
  • Peer Reviewed
• [Journal Article] Identification of the Cluster Control Region for the Protocadherin-β Genes Located beyond the Protocadherin-γ Cluster. (2011)
  • Author(s): Shinnichi Yokota, Teruyoshi Hirayama, Keizo Hirano, Ryosuke Kaneko, Shunsuke Toyoda, Yoshimi Kawamura, Masumi Hirabayashi, Takahiro Hirabayashi and Takeshi Yagi
  • Journal Title: J. Biol. Chem. Volume: 286 Pages: 31885-31895
  • Peer Reviewed
• [Journal Article] Frequent spontaneous seizures followed by spatial working memory/anxiety deficits in mice lacking sphingosine 1-phosphate receptor 2. (2011)
  • Author(s): Noriyuki Akahoshi, Yasuki Ishizaki, Hiroki Yasuda, Yoshiya L. Murashima, Toshikazu Shinba, Kaoru Goto, Toshiyuki Himi, Jerold Chun, Isao Ishii
  • Journal Title: Epilepsy & Behavior Volume: 22(4) Pages: 659-665
  • Peer Reviewed
• [Journal Article] Expression levels of Protocadherin-α transcripts are decreased by nonsense-mediated mRNA decay with frameshift mutations and by high DNA methylation in their promoter regions. (2009)
  • Author(s): Ryosuke Kaneko, Masahumi Kawaguchi, Tomoko Toyama, Yusuke Taguchi, Takeshi Yagi
  • Journal Title: Gene Volume: 430 Pages: 86-94
  • Peer Reviewed
• [Presentation] 発達期海馬長期抑圧誘発におけるカルシウムソースのスイッチ Turning on of CICR in LTD induction after critical period of NR2 subunits switch (2011)
  • Author(s): 安田 浩樹
  • Organizer: 第34回日本神経科学学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-09-16
• [Presentation] Lrfn2/SALM1 による海馬興奮性シナプスの機能制御とその遺伝子欠損マウスにみられた行動異常 (2011)
  • Author(s): 守村 直子, 安田 浩樹, 山田 一之, 富岡 直子, 片山 圭一, 山口 和彦, 太田 摩耶, 神谷 明子, 有賀 純
  • Organizer: 第34回日本神経科学学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-09-15
• [Presentation] REGULATORY ROLE OF DREBRIN IN HIPPOCAMPUSDEPENDENT LEARNING ISN-ESN-2011 (2011)
  • Author(s): Shirao, T., Hanamura, K., Yasuda, H., Kajita, Y., Kamata, Y., Sekino, Y. and Kojima, N.
  • Organizer: (International Society for Neurochemistry and European Society for Neurochemistry) 23^<rd> Biennial Meeting
  • Place of Presentation: Athens, Greece
  • Year and Date: 2011-08-30
• [Presentation] Isoform conversion of drebrin is required for hippocampus-dependent learning and synaptic plasticity in the elderly ドレブリンのアイソフォーム変換は海馬依存的学習とシナプス可塑性に必要である (2011)
  • Author(s): 児島 伸彦、安田 浩樹、花村 健次、白尾 智明
  • Organizer: 第84回日本薬理学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-03-23
• [Presentation] Neuron-specific drebrin isoform is necessary for hippocampus-dependent learning in adulthood but not in young age of mice (2010)
  • Author(s): N. KOJIMA, H. YASUDA, K. HANAMURA, T. SHIRAO
  • Organizer: Neuroscience 2010 Society for Neuroscience 40 th Annual Meeting
  • Place of Presentation: San Diego Convention Center, San Diego, USA
  • Year and Date: 2010-11-16
• [Presentation] ドレブリンアイソフォーム変換不全マウスにおける海馬シナプス可塑性と海馬依存的学習の障害 (2010)
  • Author(s): 児島 伸彦, 安田 浩樹, 花村 健次, 白尾 智明
  • Organizer: Neuro2010 第 33 回日本神経科学学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-09-04
• [Presentation] Lrfn2/SALM1, a synaptic leucine-rich repeat transmembrane molecule is required for proper synapse maturation and brain function (2009)
  • Author(s): Naoko Morimura, Maya Ota, Hiroki Yasuda, Kei-ichi Katayama, Naoko Hara, Kazuyuki Yamada, Kazuhiko Yamaguchi, Jun Aruga
  • Organizer: The 32 nd Annual Meetings of the Japan Neuroscience Society P1-b23
  • Place of Presentation: 名古屋国際会議場、Nagoya
  • Year and Date: 2009-09-17
• [Presentation] Synaptic maturation by PKN during development (2009)
  • Author(s): Hiroki Yasuda, Yishitaka Ono, Hideyuki Mukai
  • Organizer: The 32 nd Annual Meetings of the Japan Neuroscience Society P1-b23
  • Place of Presentation: 名古屋国際会議場、Nagoya
  • Year and Date: 2009-09-16
• [Presentation] SPECIFIC ROLE OF NEURONAL ISOFORM OF DREBRIN IN HIPPOCAMPAL SYNAPTIC PLASTICITY AND FEAR MEMORY (2009)
  • Author(s): Nobuhiko Kojima, Hiroki Yasuda, Kenji Hanamura, Hiroyuki Yamazaki, Tomoaki Shirao
  • Organizer: The XXXVI International Congress of Physiological Sciences (IUPS2009) P1PM-7-6 (Kyoto)
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2009-07-28
• [Book] Electrophysiological technique for analysis of synaptic function of PKN1 in hippocampus. Protein Kinase Technologies, Neuromethods vol.68, (Mukai H, eds.) (2012)
  • Author(s): Hiroki Yasuda and Hideyuki Mukai
  • Publisher: Springer, 2012