Mechanism of disease caused by deficiency of degradation of tRNA intron in mitochondria

2013 Fiscal Year Final Research Report

• Project/Area Number: 21228001
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Plant pathology
• Research Institution: Kagawa University
• Principal Investigator: AKIMITSU Kazuya 香川大学, 農学部, 教授 (80263888)
• Co-Investigator(Kenkyū-buntansha): 五味 剣二 (50511549) ; 多田 安臣 (40552740)
• Project Period (FY): 2009-05-11 – 2014-03-31
• Keywords: 農学 / 植物 / ミトコンドリア / 宿主特異的毒素 / 植物病理学

Research Abstract

ACRS gene encoding ACR-toxin receptor is degraded in mitochondria from the toxin-insensitive citrus cultivars. To identify the component proteins consisting ACRSmRNA degradation protein complex with AmBP30, which is ACRS mRNA-binding protein, yeast two hybrid with AmBP30 as the bait and immuno-precipitation using AmBP30 and Y2H-selected protein antibodies following the precipitated protein analysis by TOF-MS confirmed multiple proteins as the components of ACRSmRNA degradation protein complex. Furthermore, roles of multiple genes in ACR and ACT-toxins biosynthesis were confirmed using target-gene disruption and RNA silencing.

Research Products

• [Journal Article] Alternaria host-selective toxins : Determinant factors of plant disease (2014)
  • Author(s): Akimitsu, K., Tsuge, T., Kodama, M., Yamamoto, M., and Otani, H.
  • Journal Title: J Gen Plant Pathol Volume: 80 Pages: 109-122
• [Journal Article] zinc-binding citrus protein metallothionein can act as a plant defense factor by controlling host-selective ACR-toxin production (2013)
  • Author(s): Nishimura, S., Tatano, S., Miyamoto, Y., Ohtani, K., Fukumoto, T., Gomi, K., Tada, Y., Ichimura, K., and Akimitsu, K.
  • Journal Title: Plant Mol Biol Volume: 81 Pages: 1-11
• [Journal Article] Host-selective toxins produced by the plant pathogenic fungus Alternaria alternata (2013)
  • Author(s): Tsuge, T., Harimoto, Y., Akimitsu, K., Ohtani, K., Kodama, M., Akagi, Y., Egusa, M., Yamamoto, M., and Otani, H.
  • Journal Title: FEMS Microbiology Volume: 37 Pages: 44-66
• [Journal Article] A polyketide synthase gene, ACRTS2, is responsible for biosynthesis of host-selective ACR-toxin in the rough lemon pathotype of Alternaria alternata (2012)
  • Author(s): Izumi, Y., Ohtani, K., Miyamoto, Y., Masunaka, A., Fukumoto, T., Gomi, K., Tada, Y., Ichimura, K., Peever, T.L., and Akimitsu, K.
  • Journal Title: Mol. Plant-Microbe Interact Volume: 25 Pages: 1419-1429
• [Journal Article] Role of the pathotype-specific ACRTS1 gene encoding a hydroxylase involved in the biosynthesis of host-selective ACR-toxin in the rough lemon pathotype of Alternaria alternata (2012)
  • Author(s): Izumi, Y., Kamei, E., Miyamoto, Y., Ohtani, K., Masunaka, A., Fukumoto, T., Gomi, K., Tada, Y., Ichimura, K., Peever, T.L., and Akimitsu, K.
  • Journal Title: Phytopathology Volume: 102 Pages: 741-748
• [Journal Article] Contribution of peroxisomes to secondary metabolism and pathogenicity in the fungal plant pathogen Alternaria alternata (2010)
  • Author(s): Imazaki, A., Tanaka, A., Harimoto, Y., Yamamoto, M., Akimitsu, K., Park, P., and Tsuge, T.
  • Journal Title: Eukaryotic Cell Volume: 9 Pages: 682-694
• [Journal Article] ACTTS3 encoding a polyketide synthase is essential for the biosynthesis of ACT-toxin and pathogenicity in the tangerine pathotype of Alternaria alternata (2010)
  • Author(s): Miyamoto, Y., Masunaka, A., Tsuge, T., Yamamoto, M., Ohtani, K., Fukumoto, T., Gomi, K., Peever, T.L., Tada, Y., Ichimura, K., and Akimitsu, K.
  • Journal Title: Mol. Plant-Microbe Interact Volume: 23 Pages: 406-414
• [Journal Article] Role of the host-selective ACT-toxinsynthesis gene ACTTS2 encoding an enoyl-reductase in pathogenicity of the tangerine pathotype of Alternaria alternata (2010)
  • Author(s): Ajiro, N., Miyamoto, Y., Masunaka, A., Tsuge, T., Yamamoto, M., Ohtani, K., Fukumoto, T., Gomi, K., Peever, T.L., Izumi, Y., Tada, Y., and Akimitsu, K.
  • Journal Title: Phytopathology Volume: 100 Pages: 120-126
• [Journal Article] Molecular cloning and characterization of a thaumatin-like protein-encoding cDNA from rough lemon (2009)
  • Author(s): Kim, B.-G., Fukumoto, T., Tatano, S., Gomi, K., Ohtani, K., Tada, Y. and Akimitsu, K.
  • Journal Title: Physiol. Mol. Plant Pathol Volume: 74 Pages: 3-10
• [Journal Article] Function of genes encoding acyl-CoA synthetase and enoyl-CoA hydratase for host-selective ACT-toxin biosynthesis in the tangerine pathotype of Alternaria alternata (2009)
  • Author(s): Miyamoto, Y., Ishii, Y., Honda, A., Masunaka, A., Tsuge, T., Yamamoto, M., Ohtani, K., Fukumoto, T., Gomi, K., Peever, T.L., and Akimitsu, K.
  • Journal Title: Phytopathology Volume: 99 Pages: 369-377