Systems biology of AKT signaling

2011 Fiscal Year Final Research Report

• Project/Area Number: 21240025
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioinformatics/Life informatics
• Research Institution: The University of Tokyo
• Principal Investigator: KURODA Shinya 東京大学, 大学院・理学系研究科, 教授 (50273850)
• Project Period (FY): 2009 – 2011
• Keywords: Akt / シグナル伝達 / システム生物学 / 周波数応答 / ローパスフィルタ

Research Abstract

In cellular signal transduction, information in an external stimulus is coded as temporal patterns of signaling activities; however, the temporal coding mechanism has been poorly investigated. In this study, we modeled the epidermal growth factor (EGF)-dependent Akt pathway in PC12 cells based on experimental results, and found how the Akt pathway, which is involved in cell growth, specifically transfers temporal information of upstream signals to downstream. In addition, we theoretically analyzed a simple biochemical reaction and found that signal transfer efficiency of transient peak amplitude attenuates depending on the strength of negative regulation, and found that cells can control downstream sensitivity through attenuation of signal transfer efficiency by changing the expression level of negative regulators.

Research Products

• [Journal Article] Temporal Coding of Insulin Action through Multiplexing of the AKT Pathway (2012)
  • Author(s): Kubota, H., Noguchi, R., Toyoshima, Y., Ozaki, Y., Uda, S., Watanabe, K., Ogawa, W. and Kuroda, S.
  • Journal Title: Molecular Cell Volume: 46(6) Pages: 820-832
  • DOI: DOI:10.1016/j.molcel.2012.04.018
  • Peer Reviewed
• [Journal Article] Latent process genes for cell differentiation are common decoders of neurite extension length (2012)
  • Author(s): Watanabe, K., Akimoto, Y., Yugi, K., Uda, S., Chung, J., Nakamuta, S., Kaibuchi, K. and Kuroda, S.
  • Journal Title: Journal of Cell Science Volume: 125 Pages: 2198-2211
  • DOI: DOI:10.1242/jcs.097709
  • Peer Reviewed
• [Journal Article] Sensitivity control through attenuation of signal transfer efficiency by negative regulation of cellular signaling (2012)
  • Author(s): Toyoshima, Y., Kakuda, H., Fujita, K.,Uda, S. and Kuroda, S.
  • Journal Title: Nature.Communications Volume: 3(473)
  • DOI: DOI:10.1038/ncomms1745
  • Peer Reviewed
• [Journal Article] Decoupling of Receptor and Downstream Signals in the Akt Pathway by Its Low-Pass Filter Characteristics. (2010)
  • Author(s): Fujita, K., Toyoshima, Y., Uda, S., Ozaki, Y., Kubota, H. and Kuroda, S.
  • Journal Title: Science Signaling Volume: 3(132) Pages: 56
  • DOI: DOI:10.1126/scisignal.2000810
  • Peer Reviewed
• [Presentation] AKT経路の情報多重化によるインスリン作用の時間情報コード (2012)
  • Author(s): 久保田浩行、野口怜、豊島有、尾崎裕一、 宇田新介、渡邊可奈子、小川渉、 黒田真也
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡(福岡国際会議場)
  • Year and Date: 2012-12-12
• [Presentation] ERK経路の情報コード (2012)
  • Author(s): 黒田真也
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋(名古屋大学)
  • Year and Date: 2012-09-21
• [Presentation] Temporal Cording of Insulin Action through Multiplexing of the AKT Pathway (2012)
  • Author(s): 久保田浩行、野口怜、豊島有、尾崎裕一、 宇田新介、渡邊可奈子、小川渉、 黒田真也
  • Organizer: Joint meeting of JSDB 45th & JSCB64th
  • Place of Presentation: 神戸(神戸国際会議場)
  • Year and Date: 2012-05-29
• [Presentation] イ ンスリン作用の AKT 経路による多重情報 コード (2011)
  • Author(s): 黒田真也
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 京都(国立京都国際会館)
  • Year and Date: 2011-09-21
• [Presentation] Temporal coding of ERK and AKT signaling networks (2010)
  • Author(s): 黒田真也
  • Organizer: 2010年日本バイオインフォマティクス学会会
  • Place of Presentation: 福岡(九州大学医学部百年講堂)
  • Year and Date: 2010-12-14
• [Remarks]
  • URL: http://kurodalab.bi.s.u-tokyo.ac.jp/ja/index.html