Bioimaging of mitochondrial dynamics and generation of model mice

2011 Fiscal Year Final Research Report

• Project/Area Number: 21240044
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: 財団法人東京都医学総合研究所 (2011); Tokyo Metropolitan Organization for Medical Research (2009-2010)
• Principal Investigator: YONEKAWA Hiromichi 財団法人東京都医学総合研究所, 基盤技術研究センター, センター長 (30142110)
• Co-Investigator(Kenkyū-buntansha): SHITARA Hiroshi 財団法人東京都医学総合研究所, 基盤技術研究センター, 基盤技術研究職員 (90321885) ; KOMATSU Masaaki 財団法人東京都医学総合研究所, 生体分子先端研究分野, プロジェクトリーダー (90356254) ; KIKKAWA Yoshiaki 財団法人東京都医学総合研究所, ゲノム医科学研究分野, プロジェクトリーダー (20280787)
• Co-Investigator(Renkei-kenkyūsha): TAYA Choji 財団法人東京都医学総合研究所, 基盤技術研究センター, 室長 (90175456) ; MATSUOKA Kunie 財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主席研究員 (40291158) ; ISHII Rie 財団法人東京都医学総合研究所, 基盤技術研究センター, 主任基盤技術研究職員 (00425688) ; ONO Tomio 財団法人東京都医学総合研究所, 基盤技術研究センター, 基盤技術研究職員 (60231239)
• Project Period (FY): 2009 – 2011
• Keywords: ミトコンドリア / マウス / バイオイメージング / 疾患モデル / 運動失調

Research Abstract

To disclose the relationships between mitochondrial-dynamics' malfunctions and diseases, we tried to develop a detection systems of the malfunctions by bioimaging and to generated disease model mice caused by the malfunction. The detection systems based on bioimaging have been successfully developed by generating many lines of transgenic mice with mitochondrially expressed fluorescent proteins such as EGFP, DsRed, etc. The generation of disease model mice caused by malfunction of mitochondrial dynamics is still in progress.

Research Products

• [Journal Article] Comprehensive application of an mtDsRed2-Tg mouse strain for mitochondrial imaging (2012)
  • Author(s): Yamaguchi, J., Nishiyama, S., Shimanuki, M., Ono, T., Sato, A., Nakada, K., Hayashi, J-I., Yonekawa, H., Shitara, H
  • Journal Title: Transgenic Research Volume: 21巻 Pages: 439-447
  • DOI: DOI:10.1007/s11248-011-9539-1
  • Peer Reviewed
• [Journal Article] Generation of trans-mitochondrial mito-mice by the introduction of a pathogenic G13997A mtDNA from highly metastatic lung carcinoma cells (2010)
  • Author(s): Yokota, M., Shitara, H., Hashizume, O., Ishikawa, K., Nakada, K., Ishii, R., Taya, C., Takenaga, K., Yonekawa, H., Hayashi, J
  • Journal Title: FEBS letters Volume: 584巻 Pages: 3943-3948
  • DOI: DOI:10.1016/j.febslet.2010.07.048
  • Peer Reviewed
• [Journal Article] Global imaging of mitochondrial morphology in tissues using transgenic mice expressing mitochondrially targeted enhanced green fluorescent protein (2010)
  • Author(s): Shitara, H., Shimanuki, M., Hayashi, J-I., Yonekawa, H
  • Journal Title: Experimental Animals Volume: 59巻 Pages: 99-103
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/20224174
  • Peer Reviewed
• [Journal Article] Over-expression of Tfam improves the mitochondrial disease phenotypes in a mouse model system (2010)
  • Author(s): Nishiyama, S., Shitara, H., Nakada, K., Ono, T., Sato, A., Suzuki, H., Ogawa, T., Masaki, H., Hayashi, J-I., Yonekawa, H
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 401巻 Pages: 26-31
  • DOI: DOI:10.1016/j.bbrc.2010.08.143
  • Peer Reviewed
• [Journal Article] p62/SQSTM1 cooperates with Parkin for perinuclear clustering of depolarized mitochondria (2010)
  • Author(s): Okatsu, K., Saisho, K., Shimanuki, M., Nakada, K., Shitara, H., Sou, Y. S., Kimura, M., Sato, S., Hattori, N., Komatsu, M., Tanaka, K., Matsuda, N
  • Journal Title: Genes to cells Volume: 15巻 Pages: 887-900
  • DOI: DOI:10.1111/j.1365-2443.2010.01426
  • Peer Reviewed
• [Journal Article] New evidence confirms that the mitochondrial bottleneck is generated without reduction of mitochondrial DNA content in early primordial germ cells of mice (2009)
  • Author(s): Cao, L., Shitara, H., Sugimoto, M., Hayashi, J-I., Abe, K., Yonekawa, H
  • Journal Title: PLoS genetics Volume: 5巻 Pages: e1000756
  • DOI: DOI:10.1371/journal.pgen.1000756
  • Peer Reviewed
• [Journal Article] Mitochondrial physiology and gene expression analyses reveal metabolic and translational dysregulation in oocyte-induced somatic nuclear reprogramming
  • Author(s): Esteves, T. C., Psathaki, O. E., Pfeiffer, M. J., Balbach, S. T., Zeuschner, D., Shitara, H., Yonekawa, H., Siatkowski, M., Fuellen, G., Boiani, M
  • Journal Title: PLoS One Volume: (in press)
  • Peer Reviewed
• [Presentation] Mitochondrial imaging of in tissues by using mtDsRed2-Tg mouse strain (2011)
  • Author(s): Yamaguchi, J., Nishiyama, S., Shimanuki, M., Ono, T., Sato, A., Nakada, K., Hayashi, J-I., Yonekawa, H., Shitara, H
  • Organizer: The 10th Transgenic Technology Meeting(TT2011)
  • Place of Presentation: Florida, USA
  • Year and Date: 20111024-26
• [Presentation] ミトコンドリア転写因子A過剰発現によるミトコンドリア機能低下の抑制効果 (2011)
  • Author(s): 設楽浩志、中田和人、林純一、米川博通
  • Organizer: 第84回日本生化学会大会(シンポジウム)
  • Place of Presentation: 京都
  • Year and Date: 20110921-24
• [Presentation] マウスモデルを用いたTfam過剰発現によるミトコンドリア機能低下の抑制効果の解析 (2011)
  • Author(s): 設楽浩志、西山哲史、中田和人、小野富男、佐藤晃嗣、鈴木英紀、小川哲弘、正木晴彦、林純一、米川博通
  • Organizer: 第58日本実験動物学会総会
  • Place of Presentation: 東京
  • Year and Date: 20110525-27
• [Presentation] 遺伝子改変マウスを用いた蛍光タンパク標識によるミトコンドリアの形態学的解析 (2011)
  • Author(s): 設楽浩志
  • Organizer: 第2回サンダーバードの会(招待講演)
  • Place of Presentation: 京都
  • Year and Date: 2011-02-27
• [Presentation] Rapid segregation and bottleneck effect of mitochondrial DNA in mice (2011)
  • Author(s): Shitara, H
  • Organizer: Korea-Japan Symposium on Protein Metabolism
  • Place of Presentation: Korea
  • Year and Date: 2011-01-28
• [Presentation] The mtDNA bottleneck without reduction of its content (2010)
  • Author(s): Shitara, H
  • Organizer: 174th ENMC International Workshop "Applying Pre-implantation Genetic Diagnosis to mtDNA Diseases"
  • Place of Presentation: Naarden, the Netherlands
  • Year and Date: 20100319-22
• [Presentation] Gene therapy of mitochondrial diseases in a mouse model (2009)
  • Author(s): 設楽浩志、西山哲史、中田和人、林純一、米川博通
  • Organizer: 第32回日本分子生物学会年会.ワークショップ
  • Place of Presentation: 横浜
  • Year and Date: 20091209-12
• [Presentation] マウス初代培養細胞におけるミトコンドリア速度解析 (2009)
  • Author(s): 設楽浩志、佐藤晃嗣、島貫碧、林純一、米川博通
  • Organizer: 第55日本実験動物学会総会
  • Place of Presentation: 大宮
  • Year and Date: 20090514-16
• [Book] 外来性ミトコンドリア導入によるミトコンドリアDNAヘテロプラズミーマウス系統の樹立.生物機能モデルと新しいリソース・リサーチツール (2011)
  • Author(s): 設楽浩志
  • Total Pages: 652-655
• [Book] 卵子ミトコンドリアDNA.卵子学 (2011)
  • Author(s): 林純一、設楽浩志、佐藤晃嗣
  • Total Pages: 338-348
• [Remarks]
  • URL: http://researchmap.jp/read0002766