Biotic history has accelerated under anoxic environments - Paradox of Foraminifera evolution can solve through field observation, laboratory culture and genomic analyses.

2012 Fiscal Year Final Research Report

• Project/Area Number: 21244079
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Stratigraphy/Paleontology
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: KITAZATO Hiroshi 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 領域長 (00115445)
• Co-Investigator(Kenkyū-buntansha): TSUCHIYA Masashi 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, チームリーダー (00435835) ; OGURI Kazumasa 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 技術研究主任 (10359177) ; TOYOFUKU Takashi 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, チームリーダー (30371719) ; CHIKARAISHI Yoshito 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 主任研究員 (50455490) ; NOMAKI Hidetaka 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 研究員 (90435834)
• Project Period (FY): 2009 – 2012
• Keywords: 無酸素環境 / 真核単細胞生物 / 進化 / 適応生態 / 有孔虫

Research Abstract

We hypothesized that the evolution of marine protists might been enhanced by active microbial symbiosis. The symbiotic relationships with microbes were suitable to survive during the crisis when the anoxic environments were dominant in the ocean. The crisis is not really crisis for eukaryotic unicellular organisms because they may potentially positively adaptable to this anoxia by various types of symbiosis. We have tested the hypothesis through field surveys, accurate environment assessments, genomics, precise laboratory culture and biogeochemical measurement. Field studies were carried out at Sagami Bay, Shimoda Bay, Pacific Ocean (off Hachinohe), Namako-ike lake of Kamikoshiki-jima island, etc. The results are reported through 29 papers, 42 presentations and others for science community. Meanwhile, the knowledge has immediately transferred to society by Internet, seminars, press release and other occasions.

Research Products

• [Journal Article] Genetic diversity and environmental preferences of monothalamous foraminifers revealed through clone analysis of environmental small-subunit ribosomal DNA sequences (2013)
  • Author(s): Tsuchiya, M., Gooday, A. J., Nomaki, H., Oguri K., Kitazato H
  • Journal Title: Journal of Foraminiferal Research Volume: 43 Pages: 3-13
  • DOI: Doi:10.2113/gsjfr.43.1.3
  • Peer Reviewed
• [Journal Article] High rates of microbial carbon turnover in sediments in the deepest oceanic trench on Earth (2013)
  • Author(s): Glud Ronnie N., W. Frank , M. Mathias, Oguri,K., T. Robert, Canfield. E, Kitazato, H
  • Journal Title: Nature Geoscience Volume: 6 Pages: 284-288
  • DOI: DOI:10.1038/NGEO1773
  • Peer Reviewed
• [Journal Article] Variation in stable carbon and oxygen isotopes of individual benthic foraminifera: tracers for quantifying the vital effect (2012)
  • Author(s): Ishimura T, Tsunogai U, Hasegawa S, Nakagawa F, Oi T, Kitazato H, Suga H, Toyofuku T
  • Journal Title: Biogeosciences Discuss Volume: 9 Pages: 6191-6218
  • DOI: Doi:10.5194/bgd-9-6191-2012
  • Peer Reviewed
• [Journal Article] Low bacterial diversity and high labile organic matter concentrations in the sediments of the Medee deep-sea hypersaline anoxic basin (2012)
  • Author(s): Akoumianaki I, Nomaki H, Pachiadaki M, Kormas KA, Kitazato H, Tokuyama H
  • Journal Title: Microbes and Environments Volume: 27 Pages: 504-508
  • DOI: DOI :10.1264/jsme2.ME12045
  • Peer Reviewed
• [Journal Article] Epi-benthic megafauna zonation across an oxygen minimum zone at the Indian continental margin (2011)
  • Author(s): Hunter, W., Oguri, K., Kitazato, H., Ansari, Z.A., and Witte, U
  • Journal Title: Deep-Sea Res. I Volume: 58(6) Pages: 699-710
  • DOI: Doi:10.1016/j.dsr.2011.04.004
  • Peer Reviewed
• [Journal Article] Mg/Ca and δ18O in the brackish shallow-water benthic foraminifer Ammonia 'beccarii' (2011)
  • Author(s): Toyofuku, T., M. Suzuki, H. Suga, S. Sakai, A.Suzuki, T. Ishikawa, L. Nooijer, R. Schiebel, H. Kawahata, H. Kitazato
  • Journal Title: Marine Microplaeontology Volume: 78 Pages: 113-120
  • DOI: DOI:10.1016/j.marmicro.2010.11.003
  • Peer Reviewed
• [Journal Article] Differing utilization of dissolved and algal particulate organic matter by the deep-sea benthic ecosystem of Sagami Bay, Japan (2011)
  • Author(s): Nomaki H, Ogawa NO, Takano Y, Suga H, Ohkouchi N, and Kitazato H
  • Journal Title: Marine Ecology Progress Series Volume: 431 Pages: 11-24
  • Peer Reviewed
• [Journal Article] The role of meiofauna in deep-sea benthic food webs revealed by carbon and nitrogen stable isotope analyses (2010)
  • Author(s): Nomaki, H., N Ogawa NO, Ohkouchi N, Toyofuku T, Kitazato H
  • Journal Title: Earth, Life, and Isotopes Pages: 119-138
  • Peer Reviewed
• [Journal Article] New species of Leptohalysis, (Rhizaria, Foraminifera) from an extreme hadal site in the western Pacific Ocean (2009)
  • Author(s): Kitazato, H
  • Journal Title: Zootaxa Volume: 2059 Pages: 23-32
  • Peer Reviewed
• [Journal Article] Degradation of algal lipids by deep-sea benthic foraminifera: an in situ tracer experiment (2009)
  • Author(s): Nomaki H, Ohkouchi N, Heinz P, Suga H, Chikaraishi Y, Ogawa NO, Matsumoto K, and Kitazato H
  • Journal Title: Deep-Sea Research Part I Volume: 56 Pages: 1488-1503
  • Peer Reviewed
• [Journal Article] Foraminifera promote calcification by elevating their intracellular pH, Proc (2009)
  • Author(s): de Nooijer, L.J.,Toyofuku T., Kitazato
  • Journal Title: Nat. Ac. Sci Volume: 106 Pages: 15374-15378
  • Peer Reviewed
• [Presentation] Live (Rose-bengal stained) foraminifera from deep-sea anoxic salt brine in the Eastern Mediterranean: toward understanding limit of life for single-celled eukaryotes (foraminifera) (2012)
  • Author(s): Kitazato, H
  • Organizer: European Geophysical Union 2012 General Assembly
  • Place of Presentation: Vienna /Austria.
  • Year and Date: 20120422-27
• [Book] 日本の海はなぜ豊かなのか (2012)
  • Author(s): 北里洋
  • Total Pages: 120
  • Publisher: 岩波書店
• [Remarks]
  • URL: http://www.jamstec.go.jp/biogeos/j/elhrp/geobiology/index.html
• [Patent(Industrial Property Rights)] 光源システム及び撮像システム (2012)
  • Inventor(s): 小栗一将
  • Industrial Property Rights Holder: 独立行政法人海洋研究開発機構
  • Industrial Property Number: 特許,2012-150771, 2012
  • Filing Date: 2012-07-04