Molecular biology of microbial degradation of 2,3,7,8-TCDD for effective remediation of polychlorinated dioxins-contaminated areas.

2012 Fiscal Year Final Research Report

• Project/Area Number: 21248037
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Boundary agriculture
• Research Institution: Nihon University (2010-2012); Tokyo University of Agriculture and Technology (2009)
• Principal Investigator: KATAYAMA Yoshihiro 日本大学, 生物資源科学部, 教授 (10214339)
• Co-Investigator(Kenkyū-buntansha): NAKAMURA Masaya (独)森林総合研究所, きのこ微生物研究領域, 微生物機能解析チーム長 (50353793) ; OHTSUKA Yuichiro (独)森林総合研究所, バイオマス化学研究領域, 主任研究員 (80455261) ; TAKAHASHI Atsushi 高砂熱学工業株式会社, 常務執行役員 (60501369) ; SATO Kanna 東京農工大学, 農学研究院, 助教 (40456603) ; KAJITA Shinya 東京農工大学, 農学研究院, 准教授 (40323753)
• Project Period (FY): 2009 – 2012
• Keywords: ダイオキシン / 環境浄化 / 2,3,7,8-tetrachlorodibenzo-p-dioxin / 2,7,-dichlorodibenzo-p-dioxin / ダイオキシン蛍光アナログ / ダイオキシン還元解裂酵素 / 遺伝子ライブラリー / 遺伝子クローニング

Research Abstract

In this study, we are the first to demonstrate that Geobacillus sp. UZO3 cell free extract reductively cleavages the diaryl ether bonds of 2,3,7,8-TCDD in a sequential fashion. Furthermore, we succeeded in characterization of the gene, which contains an open reading frame of 351 bp encoding 116 amino acids. The enzyme has a CoA-binding site and reductively cleavages2,3,7,8-TCDD using CoA as the hydrogen donor.

Research Products

• [Journal Article] Develpoment of Highly Sensitive Assay for Enzyme-Mediated Reductive Degradationof Polychlorinated Dibenzo-p-dioxin (2012)
  • Author(s): Y.Suzuki, M.Nakamura, Y.Otsuka, N.Suzuki, K.Ohyama, T.Kawakami, K.Sato, S.Kajita, S.Hishiyama, T.Fujii, A.Takahashi, Y.Katayama
  • Journal Title: Environmental Toxicology and Chemistry. Volume: 31 Pages: 1072-1075
  • DOI: DOI:10.1002/etc.1775
  • Peer Reviewed
• [Journal Article] Enzymatic activity of cell free extract from Geobacillus sp.UZO 3 catalyzes reductive cleavage of diaryl ether bonds2,3,7,8-tetrachlorodibenzo- p-dioxin (2011)
  • Author(s): Y.Suzuki, M.Nakamura, Y.Otsuka, T.Kawakami, K.Sato, S.Kajita, S.Hishiyama, Y.Okamura, A.Takahashi, Y.Katayama
  • Journal Title: Organohalogen Compounds. Volume: 73 Pages: 1400-1403
  • Peer Reviewed
• [Journal Article] Novel enzymatic activity of cell free extract from thermophilic Geobacillus sp. UZO 3 catalyzes reductive cleavage of diaryl etherbonds of 2,7-dichlorodibenzo-p-dioxin (2011)
  • Author(s): Y.Suzuki, M.Nakamura, Y.Otsuka, N.Suzuki,K.Ohyama,T.Kawakami, K.Sato, S.Kajita, S.Hishiyama, T.Fujii, A.Takahashi, Y.Katayama
  • Journal Title: Chemosphere. Volume: 83 Pages: 868-872
  • DOI: DOI:10.1016/j.chemosphere.2011.
  • Peer Reviewed
• [Presentation] Microbial Degradation of 2,7-dichlorodi benzo-p-dioxin by Reductive Cleavage of Diaryl Ether Bonds (2012)
  • Author(s): Y.Otsuka, Y.Suzuki, M.Nakamura, T.Kawakami, K.Sato, S.Kajita, S.Hishiyama, A.Takahashi, Y.Katayama
  • Organizer: 32nd International Symposium on Halogenated Persistent Organic Pollutants (Dioxin 2012)
  • Place of Presentation: Cairns,Australia
  • Year and Date: 20120826-31
• [Presentation] Novel Enzymatic Activity of Cell-Free Extract from Thermophilic Geobacillus sp UZO 3 Catalyzes Reductive Cleavage of Diaryl Ether Bonds of2,7-Dichlorodi benzo-p-Dioxin (2010)
  • Author(s): M.Nakamura, Y.suzuki, Y.Otsuka, T.Kawakami, K. Sato, S.Kajita, S.Hishiyama. A.Takahashi, Y.Katayama
  • Organizer: International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2010)
  • Place of Presentation: Honollulu,USA
  • Year and Date: 20101215-20
• [Patent(Industrial Property Rights)] ダイオキシン類分解剤およびダイオキ (2012)
  • Inventor(s): 片山義博,中村雅哉,大塚裕一郎,鈴木悠造,高橋惇
  • Industrial Property Rights Holder: 日本大学、(独)森林総合研究所、高砂熱学工業株式会社、環テック株式会社
  • Industrial Property Number: 特許特願2012-53305
  • Acquisition Date: 2012-03-09