The immunopathological study on leukocyte adhesion molecule DNAM-1 (CD226)

2011 Fiscal Year Final Research Report

• Project/Area Number: 21249026
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: University of Tsukuba
• Principal Investigator: SHIBUYA Akira 筑波大学, 医学医療系, 教授 (80216027)
• Co-Investigator(Renkei-kenkyūsha): SHIBUYA Kazuko 筑波大学, 医学医療系, 准教授 (00302406) ; HONDA Shinichiro 筑波大学, 医学医療系, 准教授 (60360640) ; TAHARA Satoko 筑波大学, 医学医療系, 助教 (20360589)
• Project Period (FY): 2009 – 2011
• Keywords: CD226 / 腫瘍免疫 / 感染免疫 / 自己免疫 / DNAM-1

Research Abstract

The molecular mechanisms underlying immune responses to cancer, autoimmune diseases, and infectious diseases have been unclear. Elucidation of the molecular mechanisms is essentially required to overcome these intractable diseases. We previously identified leukocyte activating receptor DNAM-1 that is expressed on NK cells, T cell and macrophages ant its ligands. In this study, we clarify the role of DNAM-1 in the pathogenesis of diseases and develop the basis of molecular targeting therapy to these diseases.

Research Products

• [Journal Article] Identification of phosphatidylserine as a ligand for the CD300a immunoreceptor (2012)
  • Author(s): Nakahashi-Oda C, Tahara-Hanaoka S, Honda S, Shibuya K, Shibuya A
  • Journal Title: BBRC Volume: 417 Pages: 646-650
• [Journal Article] Isolation and characterization of na.ANove follicular dendritic cells (2012)
  • Author(s): Usui K, Honda S, Yoshizawa Y, Nakahashi-Oda C, Tahara-Hanaoka S, Shibuya K, Shibuya A
  • Journal Title: Mol Immunol Volume: 50 Pages: 172-6
• [Journal Article] B1b lymphocyte-derived antibodies control Borrelia hermsii independent of Fcα/μ receptor and in the absence of host cell contact (2011)
  • Author(s): Colombo MJ, Abraham D, Shibuya A, Alugupalli KR
  • Journal Title: Immunol Res Volume: 51 Pages: 249-56
• [Journal Article] The immunoreceptor adapter protein DAP12 suppresses B lymphocyte-driven adaptive immune responses (2011)
  • Author(s): Nakano-Yokomizo T, Tahara-Hanaoka S, Nakahashi-Oda C, Nabekura T, Nadia K. Tchao, Kadosaki M, Totsuka N, Kurita N, Nakamagoe K, Tamaoka A, Takai T, Yasui T, Kikutani H, Honda S, Shibuya K, Lewis L. Lanier, Shibuya A
  • Journal Title: J Exp Med Volume: 208(8) Pages: 1661-1671
• [Journal Article] Ingested allergens must be absorbed systemically to induce systemic anaphylaxis. Ingested allergens must be absorbed systemically to induce systemic anaphylaxis (2011)
  • Author(s): Strait RT, Mahler A, Hogan S, Khodoun M, Shibuya A, Finkelman FD
  • Journal Title: J Allergy ClinImmunol Volume: 127 Pages: 982-989
• [Journal Article] DNAX accessory molecule-1 (DNAM-1) plays an important role in alloreactive CD8^+ T cells responsible for the exacerbation of acute graft-versus-host disease (2011)
  • Author(s): Nabekura T, Shibuya K, Shibuya A. Replyto Seth et al
  • Journal Title: Proc Natl Acad Sci USA Volume: 108(10)E34
• [Journal Article] Critical role of DNAX accessory molecule-1 (DNAM-1) in the development of acute graft-versus-host disease in mice (2010)
  • Author(s): Nabekura T, Shibuya K, Takenaka E, KaiH, Shibata K, Yamashita Y, Harada K, Tahara-Hanaoka S, Honda S, Shibuya A
  • Journal Title: Proc Natl Acad Sci USA Volume: 107 Pages: 18593-18598
• [Journal Article] An immunoglobulin-like receptor, Allergin-1, inhibits immunoglobulin E.mediated immediate hypersensitivity reactions (2010)
  • Author(s): Hitomi K, Tahara-Hanaoka S, Someya S, Fujiki A, Tada H, Sugiyama T, Shibayama S, Shibuya K, Shibuya A
  • Journal Title: Nature Immunology Volume: 11 Pages: 601-607
• [Journal Article] Requirement of the cytoplasmic portion for dimer formation of Fcα/μ receptor expressed on cell surface (2010)
  • Author(s): Cho Y, Honda S, Yoshizawa Y, TakagakiK, Usui K, Shibuya A
  • Journal Title: Mol. Immunol Volume: 47 Pages: 878-882
• [Journal Article] Enhanced humoral immune responses against T-independent antigens in Fc α / μR-deficient mice (2009)
  • Author(s): Honda S, Miyamoto A, Cho Y, Usui K, Kurita N, Takeshita K, Takahashi S, Kinoshita T, Fujita T, Tahara-Hanaoka S, Shibuya K, Shibuya A
  • Journal Title: Proc Natl Acad Sci USA Volume: 106 Pages: 11230-11235
• [Journal Article] Regulation of immune responses by the activating and inhibitory myeloid-associate immunoglobuline-like receptors (MAIR)(CD300) (2009)
  • Author(s): Shibuya A, Nakahashi-Oda C, Tahara-Hanaoka S
  • Journal Title: Immune Network Volume: 9 Pages: 41-45
• [Journal Article] Identification of the Fca/mR isoform specifically expressed in the kidney tubule (2009)
  • Author(s): Kurita N, Honda S, Usui K, Shimizu Y, Miyamoto A, Tahara-Hanaoka S, Shibuya K, Shibuya A
  • Journal Title: Mol Immunol Volume: 46 Pages: 749-753
• [Journal Article] Receptor-mediated recognition of melanoma by NK cells and its implications for NK cell-based immunotherapy (2009)
  • Author(s): Lakshmikanth T, Burke S, Ali TH, Kimpfler S, Ursini F, Ruggeri L, Capanni M, Umansky V, Paschen A, SuckerA, Pende D, Groh V, Biassoni R, HoglundP, Kato K, Shibuya K, Schadendorf D, Anichini A, Ferrone S, Velardi A, KarreK, Shibuya A, Carbone E, Colucci F
  • Journal Title: Tissue Antigen Volume: 73 Pages: 519-520
• [Journal Article] NCRs and DNAM-1 mediate NK cell recognition and lysis of human and mouse melanoma cell lines in vitro and in vivo (2009)
  • Author(s): Lakshmikanth T, Burke S, Ali TH, Kimpfler S, Ursini F, Ruggeri L, Capanni M, Umansky V, Paschen A, SuckerA, Pende D, Groh V, Biassoni R, HoglundP, Kato K, Shibuya K, Schadendorf D, Anichini A, Ferrone S, Velardi A, Karre K, Shibuya A, Carbone E, Colucci F
  • Journal Title: J Clin Invest Volume: 119 Pages: 1251-1263
• [Presentation] Regulation of mast cell activation by inhibitory immunoglobulin-like receptors Allergin-1 and MAIR-1(CD300a) (2012)
  • Author(s): Shibuya A
  • Organizer: University Medical Center Utrecht Department of Immunology
  • Place of Presentation: Amsterdam, Netherlands
  • Year and Date: 2012-03-16
• [Presentation] Regulation of mast cell-mediated activation by inhibitory immunoreceptors (2011)
  • Author(s): Shibuya A
  • Organizer: The 3^<rd> Workshop between University of Tsukuba and University of Edinburgh
  • Place of Presentation: Edinburgh, Scotland
  • Year and Date: 20110612-15
• [Presentation] CD226 as a novel Target for Autoimmune Diseases (2011)
  • Author(s): Shibuya A
  • Organizer: Beijing Novo Nordisk Pharmaceuticals Science & Technology Co., Ltd
  • Place of Presentation: Beijing, China
  • Year and Date: 20110223-25
• [Presentation] Apoptotic cells mast cellinflammatory responses via the CD300a immunoreceptor. Harnessing Immunity to Prevent and Treat Disease meeting (2011)
  • Author(s): Shibuya A
  • Organizer: Cold Spring Harbor
  • Place of Presentation: New York, USA
  • Year and Date: 2011-11-18
• [Presentation] A novel immunoglobulin-like receptor, Allergin-1, inhibits IgE-mediated allergic responses. CSHL (2010)
  • Author(s): Satoko Tahara-Hanaoka, Kaori Hitomi, Satoru Someya, Akira Fujiki, Hideaki Tada, Tetsuya Sugiyama, Shiro Shibayama, Kazuko Shibuya, Akira Shibuya
  • Organizer: Meeting-Gene Expression & Signaling in the Immune System. Cold Spring Harbor Laboratory, Cold Spring Harbor
  • Place of Presentation: New York, USA
  • Year and Date: 20100421-25
• [Presentation] Biological function of IgMFc receptors (2010)
  • Author(s): Shibuya A
  • Organizer: 6^<th> Annual Symposium on Primary Immunodeficiency Diseases
  • Place of Presentation: California, USA
  • Year and Date: 2010-11-21
• [Presentation] Clinical Applications (2010)
  • Author(s): Shibuya A
  • Organizer: The12^<th> Meeting of the Society for Natural Immunity & NK2010
  • Place of Presentation: Dubrovnik, Croatia
  • Year and Date: 2010-09-14
• [Presentation] A critical role of paired activating and inhibitory immunoglobulin-like receptors, MAIR-I and MAIR-II, in Inflammatory responses (2010)
  • Author(s): Shibuya A
  • Organizer: The 2010 Midwinter Conference of Immunologysts at Asiloma
  • Place of Presentation: San Francisico, California, USA
  • Year and Date: 2010-01-22
• [Patent(Industrial Property Rights)] 特発性炎症性筋疾患の予防又は治療剤 (2012)
  • Inventor(s): 上阪等, 渋谷彰
  • Industrial Property Rights Holder: 上阪等, 渋谷彰
  • Industrial Property Number: 特許、2012-063595
  • Filing Date: 2012-03-21
• [Patent(Industrial Property Rights)] 臓器移植拒絶予防法の開発 (2012)
  • Inventor(s): 渋谷彰, 渋谷和子, 阿部史枝
  • Industrial Property Rights Holder: 渋谷彰, 渋谷和子, 阿部史枝
  • Industrial Property Number: 特許、2011-094
  • Filing Date: 2012-01-31
• [Patent(Industrial Property Rights)] ホスホファチジルセリン結合性物質またはCD300a結合性物質を含む免疫賦活性剤、およびホスホファチジルセリンを含む免疫抑制剤 (2011)
  • Inventor(s): 渋谷彰, 小田ちぐさ, 田原聡子
  • Industrial Property Rights Holder: 渋谷彰, 小田ちぐさ, 田原聡子
  • Industrial Property Number: 特許、2011-254151
  • Filing Date: 2011-11-21
• [Patent(Industrial Property Rights)] 移植片対宿主病予知マーカーおよびその利用 (2011)
  • Inventor(s): 渋谷彰, 小島寛
  • Industrial Property Rights Holder: 渋谷彰, 小島寛
  • Industrial Property Number: 特許、201212
  • Acquisition Date: 2011-12-21
  • Overseas
• [Patent(Industrial Property Rights)] 可溶型CD115による癌の診断とこれを標的とした癌治療 (2010)
  • Inventor(s): 渋谷彰, 渋谷和子, 井口研子
  • Industrial Property Rights Holder: 渋谷彰, 渋谷和子, 井口研子
  • Industrial Property Number: 特許、2010-17134
  • Filing Date: 2010-01-28