2011 Fiscal Year Final Research Report
Mechanisms of functional expression of pancreatic islets as an integrated system and its failure
| Project/Area Number |
21249057
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Kobe University |
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Principal Investigator |
SEINO Susumu 神戸大学, 大学院・医学研究科, 教授 (80236067)
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| Co-Investigator(Kenkyū-buntansha) |
SHIBASAKI Tadao 神戸大学, 大学院・医学研究科, 講師 (00323436)
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| Project Period (FY) |
2009 – 2011
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| Keywords | エネルギー / 糖質代謝異常 |
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| Research Abstract |
We have newly established pancreatic b-cell lines(named MIN6-K). In monolayer culture, MIN6-K8 cells respond to both glucose stimulation and cAMP-increasing agents such as the incretin hormones, while MIN6-K20 cells respond to glucose stimulation, but not to incretins. We found that the formation of three-dimensional structure of MIN6-K20 cells(pseudoislets) drastically induces cAMP/incretin responsiveness to a level similar to that of normal pancreatic islets. We also found that formation of three-dimensional structure is required for transdifferentiation of pancreatic acinar cells to b-cells in vitro. These findings indicate that three-dimensional structure of pancreatic b-cells is critical in development of normal regulation of insulin secretion as an integrative system.
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[Journal Article] Ablation of TSC2 enhances insulin secretion by increasing the number of mitochondria through activation of mTORC12011
Author(s)
Koyanagi M, Asahara S, Matsuda T, Hashimoto N, Shigeyama Y, Shibutani Y, Kanno A, Fuchita M, Mikami T, Hosooka T, Inoue H, Matsumoto M, Koike M, Uchiyama Y, Noda T, Seino S, Kasuga M, Kido Y
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Journal Title
PLoS One
Volume: 6
Pages: e23238
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[Journal Article] LKB1 regulates pancreatic beta cell size, polarity, and function2009
Author(s)
Granot Z, Swisa A, Magenheim J, Stolovich-Rain M, Fujimoto W, Manduchi E, Miki T, Lennerz JK, Stoeckert CJ, Jr., Meyuhas O, Seino S, Permutt MA, Piwnica-Worms H, Bardeesy N, and Dor Y
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Journal Title
Cell Metab
Volume: 10
Pages: 296-308
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