in situ Tissue Engineering and its application for air? way reconstruction

2011 Fiscal Year Final Research Report

• Project/Area Number: 21249074
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thoracic surgery
• Research Institution: Kyoto University
• Principal Investigator: NAKAMURA Tatsuo 京都大学, 再生医科学研究所, 准教授 (70227908)
• Co-Investigator(Renkei-kenkyūsha): IWATA Hiroo 京都大学, 再生医科学研究所, 教授 (30160120) ; TAKIGAWA Toshikazu 京都大学, 工学(系)研究科(研究院), 教授 (50201603)
• Project Period (FY): 2009 – 2011
• Keywords: ips / 複合材料 / 胸部外科 / 人工気管 / コラーゲン / 再生医学 / ビーグル犬 / 代用気管

Research Abstract

In this project, we challenged the improvement of biocompartibility of the artificial trachea using a new collagen matrix. Enhancement of epithelisation was also tried with somatic stem cells in an attempt to future clinical application. Establishment of canine iPS cells, that was the first report of the world, was achieved. MSC or autologous bone? marrow aspirate promotes the epithelisation of lumina' surface, and that the air? way of smaller diameter, such as the left main bronchus can be safely reconstructed. As a prominent spin? off of this project, the fastest method of were established making human iPS cells using a combination of 4 factors(Oct 3/4, Sox 2, Klf 4, n? Myc) and several chemical substances.

Research Products

• [Journal Article] Transforming Growth Factor/33 for the prevention of vocal fold scarring (2012)
  • Author(s): Ohno, S., Hirano, S., Kanemaru, S., Kitani, Y., Kojima, T., Ishikawa, S., Mizuta, M., Tateya, I., Nakamura, T., Ito, J.
  • Journal Title: Laryngoscope Volume: 122 Pages: 583-589
  • DOI: DOI:10.1002/lary.22389
  • Peer Reviewed
• [Journal Article] Fluid overload deteriorate the chylothorax. Disease of Esophagus (2012)
  • Author(s): Honda, M., Hori, Y., Shionoya, Y., Yamamoto, K., Kida, N., Kojima, F., Nakamura, T.
  • Journal Title: Diseases of the Esophagus Volume: 25 Pages: 269-272
  • DOI: DOI:10.1111/j.1442-2050.2011.01237.xl
  • Peer Reviewed
• [Journal Article] Accelerated generation of human induced pluripotent stem cells with retroviral transduction and chemical inhibitors under physiological hypoxia (2012)
  • Author(s): Shimada, H., Hashimoto, Y., Nakada, A., Shigeno, K., Nakamura, T.
  • Journal Title: BBRC Volume: 417 Pages: 659-664
  • DOI: DOI:10.1016/j.bbrc.2011.11.111
  • Peer Reviewed
• [Journal Article] Reduction of air leaks in a canine model of pulmonary resection with a new staple-line buttress (2011)
  • Author(s): Hashimoto, A., Kuwabara, M., Hirasaki, Y., Tsujimoto, H., Torii, T., Nakamura, T., Hagiwara, A.
  • Journal Title: J Thorac Cardiovasc Surg Volume: 142 Pages: 366-371
  • DOI: DOI:10.1016/j.jtcvs.2011.05.001
  • Peer Reviewed
• [Journal Article] The protective efficacy of basic fibroblast growth factor in radiation-induced salivary gland dysfunction in mice (2011)
  • Author(s): Kojima, T., Kanemaru, S., Hirano, S., Tateya, I., Suehiro, A., Kitani, Y., Kishimoto, Y., Ohno, S., Nakamura, T., Ito, J.
  • Journal Title: Laryngoscope Volume: 121 Pages: 1870-1875
  • DOI: DOI:10.1002/lary.21873
  • Peer Reviewed
• [Journal Article] Regeneration of radiation damaged salivary glands with adipose-derived stromal cells (2011)
  • Author(s): Kojima, T., Kanemaru, S., Hirano, S., Tateya, I., Ohno, S., Nakamura, T., Ito, J.
  • Journal Title: Laryngoscope Volume: 121 Pages: 1864-1869
  • DOI: DOI:10.1002/lary.22080
  • Peer Reviewed
• [Journal Article] Development of a novel ant adhesive material, alginate flakes, ex vivo and in vivo (2011)
  • Author(s): Hirasaki, Y., Fukunaga, M., Kidokoro, A., Hashimoto, A., Nakamura, T., Tsujimoto, H., Hagiwara, A.
  • Journal Title: Surg today Volume: 41 Pages: 970-977
  • DOI: DOI:10.1007/s00595-010-4410-1
  • Peer Reviewed
• [Journal Article] Meta-analysis of therapeutic procedures for acquired subglottic stenos is in adults (2011)
  • Author(s): Yamamoto, K., Kojima, F., Tomiyama, K., Nakamura, T., Hayashino, Y.
  • Journal Title: Ann Thorac Surg Volume: 91 Pages: 1747-1753
  • DOI: DOI:10.1016/j.athoracsur.2011.02.071
  • Peer Reviewed
• [Journal Article] Use of adipose-derived stoma cells for prevention of esophageal stricture after circumferential EMR in a canine model (2011)
  • Author(s): Honda, M., Hori, Y., Nakada, A., Uji, M., Nishizawa, Y., Yamamoto, K., Kobayashi, T., Shimada, H., Kida, N., Sato, T., Nakamura, T.
  • Journal Title: Gastrointestinal Endoscopy Volume: 73 Pages: 777-784
  • DOI: DOI:10.1016/j.gie.200.11.008
  • Peer Reviewed
• [Journal Article] Feasibility study of corticosteroid treatment for esophageal ulcer after EMR in a canine model (2011)
  • Author(s): Honda, M., Nakamura, T., Hori, Y., Shionoya, Y., Yamamoto, K., Nishizawa, Y., Kojima, F., Shigeno, K.
  • Journal Title: Journal of Gastroenterology Volume: 46 Pages: 866-872
  • DOI: DOI:10.1007/s00535-011-0400-3
  • Peer Reviewed
• [Journal Article] Prevention of buccal mucosa scarring with transforming growth factor 3 3 (2011)
  • Author(s): Ohno, S., Hirano, S., Kanemaru, S., Tateya, I., Kitani, Y., Kojima, T., Nakamura, T., Ito, J.
  • Journal Title: Laryngoscope Volume: 121 Pages: 1404-1409
  • DOI: DOI:10.1002/lary.21759
  • Peer Reviewed
• [Journal Article] Implantation of an atelocollagen sponge with autologous bone marrow-derived mesenchymal stromal cells for treatment of vocal fold scarring in a canine model (2011)
  • Author(s): Ohno, S., Hirano, S., Kanemaru, S., Kitani, Y., Kojima, T., Tateya, I. Nakamura, T., Ito, J.
  • Journal Title: Ann Otol Rhinol Laryngol Volume: 120 Pages: 401-408
  • URL: http://search.proquest.com/docview/902095273/136F778BB261405ED68/1?accountid=11929
  • Peer Reviewed
• [Journal Article] Tissue-engineered airway and "in situ tissue engineering" (2011)
  • Author(s): Nakamura, T., Omori, K., Kanemaru, S.
  • Journal Title: Gen Thorac Cardiovasc Surg Volume: 59 Pages: 91-97
  • DOI: DOI:10.1007/s11748-010-0677-9
  • Peer Reviewed
• [Journal Article] 肺における再生医療 (2011)
  • Author(s): 中村達雄
  • Journal Title: Clinical Engineering Volume: 22 Pages: 32-36
• [Journal Article] Glottal reconstruction with a tissue engineering technique using polypropylene mesh : a canine experiment (2010)
  • Author(s): Yamashita, M., Kanemaru, S., Hirano, S., Umeda, H., Kitani, Y., Omori, K., Nakamura, T., Ito, J.
  • Journal Title: Ann Otol Rhinol Laryngol Volume: 119(2) Pages: 110-117
  • URL: http://search.proquest.com/docview/217943107/fulltextPDF?accountid=11929
  • Peer Reviewed
• [Journal Article] Process of healing of mucosal defects in the esophagus after endoscopic mucosal resection : histological evaluation in a dog model. Endoscopy (2010)
  • Author(s): Honda, M., Nakamura, T., Hori, Y., Shionoya, Y., Nakada, A., Sato, T., Yamamoto, K., Kobayashi, T., Shimada, H., Kida, N., Hashimoto, A., Hashimoto, Y.
  • Journal Title: Endoscopy Volume: 42 Pages: 1092-1095
  • Peer Reviewed
• [Journal Article] Observation of the esophagus, pharynx and lingual root by gastrointestinal endoscopy with a percutaneous retrograde approach (2010)
  • Author(s): Honda, M., Hori, Y., Shionoya, Y., Nakada, A., Sato, T., Kobayashi, T., Shimada, H., Kida, N., Nakamura, T.
  • Journal Title: World J Gastrointest Endosc Volume: 16 Pages: 288-292
  • DOI: DOI:10.4253/wjge.v2.i8.288
  • Peer Reviewed
• [Journal Article] Treatment of acute vocal fold scar with local injection of basic fibroblast growth factor : a canine study (2010)
  • Author(s): Suehiro, A., Hirano, S., Kishimoto, Y., Rousseau, B., Nakamura, T., Ito, J.
  • Journal Title: Acta Otolaryngol Volume: 130 Pages: 844-850
  • DOI: DOI:10.3109/00016480903426618
  • Peer Reviewed
• [Journal Article] Generation of canine induced pluripotent stem cells by retroviral transduction and chemical inhibitors (2010)
  • Author(s): Shimada, H., Nakada, A., Hashimoto, Y., Shigeno, K., Shionoya, Y., Nakamura, T.
  • Journal Title: Molecular Reproduction and Development Volume: 77 Pages: 2
  • DOI: DOI:10.1002/mrd.21117
  • Peer Reviewed
• [Journal Article] Biodegradable polymer coating promotes the epithelization of tissue-engineered airway prostheses (2010)
  • Author(s): Sato, T., Araki, M., Nakajima, N., Omori, K., Nakamura, T.
  • Journal Title: J Thorac Carciovasc Surg Volume: 139 Pages: 26-31
  • DOI: DOI:10.1016/j.jtcvs.2009.04.006
  • Peer Reviewed
• [Journal Article] A tissue-engineered trachea derived from a framed collagen scaffold gingival fibroblasts and adipose-derived stem cells (2010)
  • Author(s): Kobayashi, K., Suzuki, T., Nomoto, Y., Tada, Y., Miyake, M., Hazama, A., Wada, I., Nakamura, T., Omori, K.
  • Journal Title: Biomaterials Volume: 31 Pages: 4855-4863
  • DOI: DOI:10.1016/j.biomaterials.2010.02.027m4)-
  • Peer Reviewed
• [Journal Article] Intratracheal catheter suction removes the same volume of me conium with less impact on desaturatio compared with me conium aspirator in me conium aspiration syndrome (2010)
  • Author(s): Akazawa, Y., Ishida, T., Baba, A., Hiroma, T., Nakamura, T.
  • Journal Title: Early Hum Dev. Volume: 86 Pages: 499-502
  • DOI: DOI:10.1016/j.earlhumdev.2010.06.011
  • Peer Reviewed
• [Journal Article] Potential of induced pluripotent stem cells for the regeneration of the tracheal wall (2010)
  • Author(s): Imaizumi, M., Nomoto, Y., Sugino, T., Miyake, M., Wada, I., Nakamura, T., Omori, K.
  • Journal Title: Ann Otol Rhinol Laryngol Volume: 119 Pages: 697-703
  • URL: http://search.proquest.com/docview/759349417/fulltextPDF?accountid=11929
  • Peer Reviewed
• [Journal Article] A tissue-engineering approach for stenos is of the trachea and/or cricoid (2010)
  • Author(s): Kanemaru, S., Hirano, S., Umeda, H., Yamashita, M., Suehiro, A., Nakamura, T., Maetani, T., Omori, K., Ito, J.
  • Journal Title: Acta Otolaryngol Suppl Volume: 130 Pages: 79-83
  • DOI: DOI:10.3109/00016489.2010.496462
  • Peer Reviewed
• [Journal Article] 人工神経の基礎と臨床 (2010)
  • Author(s): 中村達雄
  • Journal Title: Peripheral Nerve Volume: 21 Pages: 192-196
• [Journal Article] in situ Tissue Engineeringを用いた気管・気管支の再建 (2010)
  • Author(s): 中村達雄、大森孝一、金丸眞一
  • Journal Title: 日本気管食道科学会会報 Volume: 61 Pages: 89
• [Journal Article] Regeneration of central nervous tissue using a collagen scaffold and adipose? derived stromal cells (2009)
  • Author(s): Nakada, A., Fukuda, S., Ichihara, S., Sato, T., Itoi, S., Inada, Y., Endo, K., Nakamura, T.
  • Journal Title: Cells Tissues Organs Volume: 190 Pages: 326-335
  • DOI: DOI:10.1159/00022323
  • Peer Reviewed
• [Journal Article] Effect of exogenous hepatocyte growth factor on vocal fold fibroblasts (2009)
  • Author(s): Kishimoto, Y., Hirano, S., Suehiro, A., Tateya, I., Kanemaru, S., Nakamura, T., Ito, J.
  • Journal Title: Ann Otol Rhinol Laryngol Volume: 118 Pages: 606-611
  • URL: http://search.proquest.com/docview/217912014/fulltextPDF?accountid=11929
  • Peer Reviewed
• [Journal Article] Development of a new tissue? engineered sheet for reconstruction of the stomach (2009)
  • Author(s): Araki, M., Tao, H., Sato, T., Nakajima, N., Hyon, Nagayasu, T., Nakamura, T.
  • Journal Title: Artificial Organs Volume: 33 Pages: 818-826
  • DOI: DOI:10.1111/j.1525-1594.2009.00808.
  • Peer Reviewed
• [Journal Article] 気道の再互瀦一 (2009)
  • Author(s): 中村達雄、大森孝一、金丸眞一
  • Journal Title: 治療学 Volume: 43 Pages: 49-52
• [Journal Article] In situ Tissue Engineering for tracheal reconstruction using a luminar remodeling type of artificial trachea (2009)
  • Author(s): Nakamura, T., Sato, T., Araki, M., Ichihara, S., Nakada, A., Yoshitani, M., Itoi, S., Yamashita, M., Kanemaru, S., Omori, K., Hori, Y., Endo, K., Inada, Y., Hayakawa, K.
  • Journal Title: J Thorac Cardiovasc Surg Volume: 138 Pages: 811-819
  • DOI: DOI:10.1016/j.jtcvs.2008.07.072
  • Peer Reviewed
• [Presentation] ステッピングモータによる臓器硬さ自動計測システムの開発 (2011)
  • Author(s): 西尾建佑、河合俊和、西澤祐吏、中村達雄
  • Organizer: 日本コンピュータ外科学
  • Place of Presentation: 東京
  • Year and Date: 20111122-24
• [Presentation] Utility of a Slightly Modified Collagen Scaffold (2011)
  • Author(s): Nakada, A., Shigeno, K.
  • Organizer: Bio Japan 2011 World Business Forum
  • Place of Presentation: Yokohama
  • Year and Date: 20111005-07
• [Presentation] Measurement method for internal organs elasticity from step out phenomenon of stepper motor (2011)
  • Author(s): Kawai, T., Mizuno, S., Nishizawa, Y., Nakamura, T.
  • Organizer: Int J CARS Computer Assist Radiol Surg
  • Place of Presentation: Berlin(Germany)
  • Year and Date: 20110622-25
• [Presentation] イヌips細胞から神経堤細胞への分化誘導 (2011)
  • Author(s): 島田英徳、橋本典也、中田顕、茂野啓示、中村達雄
  • Organizer: 第十回日本再生医療学会
  • Place of Presentation: 東京
  • Year and Date: 20110301-02
• [Presentation] 肛門機能の再生に関する研究 (2011)
  • Author(s): 西澤祐吏、中村達雄
  • Organizer: 雅度京都大学再生研若手発表会
  • Place of Presentation: 京都
  • Year and Date: 2011-12-26
• [Presentation] 内視鏡下手術支援ロボットLODEMを用いた腹腔鏡下手術コンセプト (2011)
  • Author(s): 西澤祐吏、中村達雄、河合俊和、橋田淳、申明奎
  • Organizer: 日本内視鏡外科学会
  • Place of Presentation: 大阪
  • Year and Date: 2011-12-07
• [Presentation] Implantation of an Atelocollagen Sponge with Bone Marrow-Derived Stromal Cells for the Treatment of Vocal Fold Scarring (2011)
  • Author(s): Ohno, S., Hirano, S., Kanemaru, S., Kitani, Y., Kojima, T., Tateya, I., Nakamura, T., Ito, J.
  • Organizer: The 91st Annual Meeting of The American Broncho-Esophagological Association
  • Place of Presentation: Chicago(USA)
  • Year and Date: 2011-04-28
• [Presentation] 生体内再生(insitu Tissue Engineering)と末梢神経の再生医療 (2011)
  • Author(s): 中村達雄
  • Organizer: 第11回長崎障害者支援再生医療研究会
  • Place of Presentation: 長崎
  • Year and Date: 2011-04-26
• [Presentation] Reprogramming of canine adult somatic cells to induced pluripotent stem cells with retroviral transduction and chemical inhibitors under hypoxic conditions (2010)
  • Author(s): Shimada, H., Nakada, A., Hashimoto, Y., Shigeno, K., Nakamura, T.
  • Organizer: ISSCK(国際幹細胞学会)
  • Place of Presentation: San Franciso(USA)
  • Year and Date: 20100616-19
• [Presentation] Autologous transplantation of adipose-derived stromal cells prevent esophageal stricture after endoscopic mucosal resection in a canine model (2010)
  • Author(s): Honda, M., Hori, Y., Nakada, A., Uji, M., Nishizawa, Y., Yamamoto, K., Kobayashi, T., Shimada, H., Kida, N., Sato, T., Nakamura, T.
  • Organizer: 第63回日本胸部外科学会
  • Place of Presentation: 大阪
  • Year and Date: 2010-10-26
• [Presentation] 食道ESD後の創傷治癒過程と狭窄予防に対する再生医療の応用.シンポジウム「EMR、ESDの課題安全確実な内視鏡医療の提供に向けて (2010)
  • Author(s): 本多通孝、中田顕、中村達雄
  • Organizer: 第18回日本消化器関連学会週間
  • Place of Presentation: 横浜
  • Year and Date: 2010-10-15
• [Presentation] 食道内視鏡治療後の狭窄予防にステロイド、マイトマイシンCは有効か (2010)
  • Author(s): 本多通孝、中村達雄、山本一道、堀義生、中田顕、塩谷伊毅、小林丈士、島田英徳、木田直樹
  • Organizer: 第18回日本消化器関連学会週間
  • Place of Presentation: 横浜
  • Year and Date: 2010-10-14
• [Presentation] アルコールブロック後のイヌ星状神経節の組織学的検討とホルネル徴候の変化 (2010)
  • Author(s): 塩谷伊毅、砂田勝久、中村達雄
  • Organizer: 第38回日本歯科麻酔学会総会・学術集会(ボスター)
  • Place of Presentation: 横須賀
  • Year and Date: 2010-10-09
• [Presentation] Transplantation of adipose-derived stromal cells prevent esophageal stricture after endoscopic mucosal resection in canine model (2010)
  • Author(s): Honda, M., Hori, Y., Nakada, A., Uji, M., Nishizawa, Y., Yamamoto, K., Kobayashi, T., Shimada, H., Kida, N., Sato, T., Nakamura, T.
  • Organizer: European Society for Artificial Organs 37th Congress
  • Place of Presentation: Skopje(Macedonia)
  • Year and Date: 2010-09-10
• [Presentation] 人工神経の基礎と臨床 (2010)
  • Author(s): 中村達雄、萩原明於、稲田有史、金丸眞一
  • Organizer: 第21回末梢神経学会学術集会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-04
• [Presentation] 脂肪由来幹細胞を用いた食道ESD後狭窄の予防 (2010)
  • Author(s): 本多通孝、堀義生、中村達雄、山本一道、中田顕、塩谷伊毅、小林丈士、島田英徳、木田直樹
  • Organizer: 第64回日本食道学会
  • Place of Presentation: 久留米
  • Year and Date: 2010-09-01
• [Presentation] Generation of canine induced pluripotent stem cells (2009)
  • Author(s): Shimada, H., Nakada, A., Hashimoto, Y., Shigeno, K., Shionoya, Y., Kida N., Honda M., Nakamura, T.
  • Organizer: Reprogramming Cell Fate Basic Biology and Medical Perspectives
  • Place of Presentation: Milano(Italy)
  • Year and Date: 20091211-13
• [Presentation] 脂肪組織由来幹細胞とコラーゲンの足場を用いたinsituTissueEngineeringと中枢神経再生の試み (2009)
  • Author(s): 中田顕、稲田有史、遠藤克昭、中村達雄
  • Organizer: 第31回日本バイオマテリアル学会大会
  • Place of Presentation: 京都
  • Year and Date: 20091116-17
• [Presentation] insituTissueEngineeringを用いた気管・気管支の再建 (2009)
  • Author(s): 中村達雄
  • Organizer: 第61回日本気管食道科学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20091105-06
• [Presentation] 肝切離面に対する新しい癒着匡禰開発 (2009)
  • Author(s): 荒木政人、中島直喜、須賀井一、玄丞焦、七島篤志、日高重和、澤井照光、國崎真己、飛永修一、永安武、中村達雄
  • Organizer: 第109回日本外科学会総会
  • Place of Presentation: 福岡
  • Year and Date: 20090402-04
• [Presentation] 医工学の融合による新規医療材料の開発と再生医療 (2009)
  • Author(s): 荒木政人、中村達雄、中島直喜、須賀井一、玄丞娠一高天克典、永安武
  • Organizer: 第4回長崎障害者支援再生医療研究会
  • Place of Presentation: 長崎
  • Year and Date: 2009-09-29
• [Presentation] Polyglycolicacid-collagen「蔽「で修復したイヌ下歯槽神経の再生に星状神経節ブロックが及ぼす影響 (2009)
  • Author(s): 塩谷伊毅、砂田勝久、松野智宣、佐藤田鶴子、中村達雄
  • Organizer: 平成21年度日本歯科大学歯学大会・総会
  • Place of Presentation: 新潟
  • Year and Date: 2009-06-06
• [Presentation] lnsitu Tissue Engineering(生体内再生医療)の整形外科領域への臨床応用 (2009)
  • Author(s): 中村達雄
  • Organizer: 第27回松戸整形外科医会教育研修講演会
  • Place of Presentation: 松戸
  • Year and Date: 2009-05-27
• [Presentation] ips細胞研究の展望と問題点.福県立医科大学医学部耳鼻咽喉科学講座 (2009)
  • Author(s): 中村達雄
  • Organizer: 第12回耳鼻咽喉科再生医学研究会
  • Place of Presentation: 福島
  • Year and Date: 2009-04-20
• [Book] 食道・気管.遺伝子医学MOOK13恵者までとどいている再生誘導治療 (2009)
  • Author(s): 中村達雄
  • Total Pages: 308
  • Publisher: 株式会社メディカルドゥ
• [Remarks]
  • URL: http://www.frontier.kyoto-u.ac.jp/caO4/index.htm1