Elucidation of MHC polymorphism information in cynomolgus macaque(Macaca Fascicularis)

2011 Fiscal Year Final Research Report

• Project/Area Number: 21300155
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Tokai University
• Principal Investigator: SHHNA Takashi 東海大学, 医学部, 准教授 (00317744)
• Co-Investigator(Kenkyū-buntansha): ANDO Asako 東海大学, 医学部, 准教授 (40101935) ; TORII Ryuzo 滋賀医科大学, 動物生命科学研究センター, 教授 (50106647) ; TSUCHIYA Hideaki 滋賀医科大学, 動物生命科学研究センター, 助教 (10378440) ; ITOH Yasushi 滋賀医科大学, 医学部, 准教授 (90324566) ; OGASAWARA Kazumasa 滋賀医科大学, 医学部, 教授 (20169163) ; HOSOMICHI Kazuyoshi 国立遺伝学研究所, 総合遺伝研究系人類遺伝研究部門, 助教 (50420948) ; OTA Masao 信州大学, 医学部, 准教授 (50115333)
• Project Period (FY): 2009 – 2011
• Keywords: 遺伝育種

Research Abstract

We developed a genotyping methods on Macaca fascicularis(Mafa) class I and class II genes by Sanger and next generation sequencing. Using the methods we discovered new MHC alleles, and estimated MHC haplotypes in Filipino population from genotyping of the 200 animals. Moreover, we characterized five homozygous and 43 heterozygous animals that have the most frequent MHC haplotype and 23 homozygous animals that have the other MHC haplotypes from a large scale screening of 1222 animals. In summary, we characterized the Macaca fascicularis MHC polymorphisms and constructed research base for medical research in this research.

Research Products

• [Journal Article] Memory Immune Responses Against Pandemic 1(H1N1) 2009 Influenza Virus Induced by a Whole Particle Vaccine in Cynomolgus Monkeys Carrying Mafa-A1 052 : 02 (2012)
  • Author(s): Arikata M, Itoh Y, Okamatsu M, Maeda T, Shiina T, Tanaka K, Suzuki S, Nakayama M, Sakoda Y, Ishigaki H, Takada A, Ishida H, Soda K, Pham VL, Tsuchiya H, Nakamura S, Torii R, Shimizu T, Inoko H, Ohkubo I, Kida H, Ogasawara K
  • Journal Title: PLoS ONE Volume: 7 Pages: e37220
  • Peer Reviewed
• [Journal Article] Establishment of exome sequencing of cynomolgus macaque(Macaca fascicularis) (2011)
  • Author(s): Suzuki S, Hosomichi K, Otabe K, Sakamoto K, Kurata M, Nomura M, Yamanaka H, Nakagawa H, Ota M, Inoko H, Inoue I, Shiina T
  • Journal Title: DNA Polymorphism Volume: 19 Pages: 230-234
• [Journal Article] Analysis of MHC class II gene polymorphisms(Mafa-DR-DQ genes) in Cynomolgus Macaques(Macaca fascicularis) originated from Philippine islands (2011)
  • Author(s): Ota M, Tanaka T, Katsuyama Y, Kita Y, Otabe K, Sakamoto K, Kurata M, Nomura M, Yamanaka H, Nakagawa H, Asamura H, Inoko H, Shiina T
  • Journal Title: DNA Polymorphism Volume: 19 Pages: 47-49
• [Journal Article] Study of Cynomolgus monkey(Macaca fascicularis) DRA polymorphism in four populations (2010)
  • Author(s): Aarnink A, Estrade L, Apoil PA, Kita YF, Saitou N, Shiina T, Blancher A
  • Journal Title: Immunogenetics Volume: 62 Pages: 123-136
  • Peer Reviewed
• [Journal Article] Mitochondrial DNA diversity among three subpopulations of cynomolgus macaques(Macaca fascicularis) originating from the Indochinese region (2010)
  • Author(s): Shiina T, Tanaka K, Katsuyama Y, Otabe K, Sakamoto K, Kuratani M, Nomura M, Yamanaka H, Nakagawa H, Inoko H, Ota M
  • Journal Title: Exp. Anim Volume: 59 Pages: 567-578
  • Peer Reviewed
• [Journal Article] MHC class I A loci polymorphism and diversity in three Southeast Asian populations of cynomolgus macaque (2009)
  • Author(s): Kita YF, Hosomichi K, Kohara S, Itoh Y, Ogasawara K, Tsuchiya H, Torii R, Inoko H, Blancher A, Kulski JK, Shiina T
  • Journal Title: Immunogenetics Volume: 61 Pages: 635-648
  • Peer Reviewed
• [Journal Article] Nomenclature report on the major histocompatibility complexes, genes, and alleles of non-human primate species
  • Author(s): Groot NG, Otting N, Robinson J, Blancher A, Lafont BA, Marsh SGE, 0' Connor DH, Shiina T, Walter L, Watkins DI, Bontrop RE
  • Journal Title: Immunogenetics Volume: (In press)
  • Peer Reviewed
• [Journal Article] Study of cynomolgus monkey(Macaca fascicularis) MhcDRB polymorphism in four populations
  • Author(s): Blancher A, Aarnink A, Tanaka K, Ota M, Inoko H, Yamanaka H, Nakagawa H, Apoil PA, Shiina T
  • Journal Title: Immunogenetics Volume: (In press)
  • Peer Reviewed
• [Presentation] カニクイザルMHC多型情報基盤と生物医学研究への展開、シンポジウムI「MHCからみた種内多様性と進化」 (2011)
  • Author(s): 椎名隆
  • Organizer: 第20回日本組織適合性学会大会
  • Place of Presentation: ツインメッセ静岡
  • Year and Date: 2011-08-29
• [Presentation] Elucidation of Genomic Structure and Diversity of the Macaca Fascicularis MHC (2011)
  • Author(s): Shiina T, Suzuki S, Tanaka K, Yamanaka H, Nakagawa H, Ota M, Torii R, Ogasawara K, Inoko H, Blancher A
  • Organizer: SMBE2011
  • Place of Presentation: Kyoto University
  • Year and Date: 2011-07-27
• [Presentation] 次世代シークエンサーを用いたカニクイザルにおける薬物代謝関連遺伝子内SNP検出 (2011)
  • Author(s): 鈴木進悟、小田部耕二、坂本憲吾、倉田昌明、野村護、山中久、中川博司、太田正穂、猪子英俊、椎名隆
  • Organizer: 第58回日本実験動物学会総会
  • Place of Presentation: タワーホール船堀
  • Year and Date: 2011-05-25
• [Presentation] 次世代シークエンサーを用いたカニクイザルにおけるExome変異検出法の確立 (2010)
  • Author(s): 鈴木進悟、細道一善、小田部耕二、坂本憲吾、倉田昌明、野村護、山中久、中川博司、太田正穂、猪子英俊、井ノ上逸朗、椎名隆
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 三島市民文化会館・ゆうゆうホール
  • Year and Date: 2010-11-18
• [Presentation] 次世代シークエンサーを用いたカニクイザルにおけるExome変異検出法の確立 (2010)
  • Author(s): 鈴木進悟、細道一善、小田部耕二、坂本憲吾、倉田昌明、野村護、山中久、中川博司、太田正穂、猪子英俊、井ノ上逸朗、椎名隆
  • Organizer: 日本DNA多型学会第19回学術集会
  • Place of Presentation: 三島市民文化会館・ゆうゆうホール
  • Year and Date: 2010-11-18
• [Presentation] 生物医学研究推進のためのフィリピン産カニクイザルMHC遺伝子の多型解析 (2010)
  • Author(s): 太田正穂、田中景子、勝山善彦、北夕紀、小田部耕二、坂本憲吾、倉田昌明、野村護、山中久、中川博司、浅村英樹、猪子英俊、椎名隆
  • Organizer: 第19回DNA多型学会
  • Place of Presentation: 三島市民文化会館・ゆうゆうホール
  • Year and Date: 2010-11-18
• [Presentation] MHC全遺伝子ホモ接合体カニクイザルの特定 (2010)
  • Author(s): 椎名隆、北夕紀、田中景子、河野あづみ、鈴木進悟、細道一善、勝山善彦、土屋英明、小田部耕二、坂本憲吾、倉田昌明、野村護、山中久、中川博司、伊藤靖、太田正穂、猪子英俊、鳥居隆三、小笠原一誠
  • Organizer: 第19回日本組織適合性学会大会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-09-18
• [Presentation] カニクイザル3集団におけるMHCクラスI遺伝子(Mafa-A)の多型性の比較 (2009)
  • Author(s): 北夕紀、田中景子、細道一善、小笠原一誠、鳥居隆三、猪子英俊、椎名隆
  • Organizer: 第18回日本組織適合性学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-09-26
• [Patent(Industrial Property Rights)] カニクイザルMHC全遺伝子ホモ接合体を作成するためのキット及び方法 (2011)
  • Inventor(s): 小笠原一誠、鳥居隆三、椎名隆
  • Industrial Property Rights Holder: 学校法人東海大学、国立大学法人滋賀医科大学
  • Industrial Property Number: 特許、 PCT/JP2011/060233
  • Filing Date: 2011-05-02
  • Overseas