2012 Fiscal Year Final Research Report
Risk assessment of environmental oxidative chemicals using catalase mutant primary cultured hepatocytes
| Project/Area Number |
21310022
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental impact assessment/Environmental policy
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| Research Institution | Okayama University |
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Principal Investigator |
WANG Da-hong 岡山大学, 大学院・医歯薬学総合研究科, 助教 (90294404)
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| Co-Investigator(Kenkyū-buntansha) |
FUJITA Hirofumi 岡山大学, 大学院・医歯薬学総合研究科, 助教 (20423288)
OGINO Keiki 岡山大学, 大学院・医歯薬学総合研究科, 教授 (70204104)
TSUTSUI Ken 岡山大学, 自然生命科学研究支援センター, 教授 (70108158)
SANO Kuniaki 岡山大学, 大学院・医歯薬学総合研究科, 助教 (00294405)
NAKAMURA Kazuyuki 山口大学, 医学(系)研究科, 教授 (90107748)
MIYAZAKI Masahiro 岡山学院大学, 人間生活学部, 教授 (90116509)
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| Co-Investigator(Renkei-kenkyūsha) |
TSUTSUI Ken 岡山大学, 自然生命科学研究支援センター, 教授 (70108158)
SANO Kuniaki 岡山大学, 大学院・医歯薬学総合研究科, 助教 (00294405)
NAKAMURA Kazuyuki 山口大学, 医学(系)研究科, 教授 (90107748)
MIYAZAKI Masahiro 岡山学院大学, 人間生活学部, 教授 (90116509)
MASUOKA Noriyoshi 岡山理科大学, 理学部, 教授 (20116502)
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| Project Period (FY) |
2009 – 2012
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| Keywords | 初代培養肝細胞 / カタラーゼ / 過酸化水素 / ヒドロキノン / アポトーシス / ローソン / レスベラトロール |
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| Research Abstract |
We exposured mouse primary hepatocytes to chemicals that are commonly used in our daily lives like hydroquinone, lawsone. We found that exposure the cells to these chemicals decreased cellular viability in both catalase mutant mice (Cs^b) and the wild-type mice (Cs^a) dose-dependently, in particular, the survival of hepatocytes was lower in Csbwhen compared to that of Cs^a. We also observed that exposure the cells to hydroquinone, a main component of skin-lightening cream, enhanced the cellular expression of cytochrome P450 2E1 mRNA and protein dose-dependently as well as induced apoptosis of the cells.
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[Journal Article] Acatalasemic mice are mildly susceptible to adriamycin nephropathy and exhibit increased albuminuria and glomerulosclerosis2012
Author(s)
Takiue K, Sugiyama H, Inoue T, Morinaga H, Kikumoto Y, Kitagawa M, Kitamura S, Maeshima Y, Wang DH, Masuoka N, Ogino K, Makino H
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Journal Title
BMC Nephrol.
Volume: 13
Pages: 14
Peer Reviewed
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[Journal Article]2010
Author(s)
Kikumoto Y, Sugiyama H, Inoue T, Morinaga H, Takiue K, Kitagawa M, Fukuoka N, Saeki M, Maeshima Y, Wang DH, Ogino K, Masuoka N, Makino H
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Journal Title
Biochim. Biophys. Acta
Volume: 1802
Pages: 240-246
Peer Reviewed
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